Chromosomal rearrangements and instability caused by the LINE-1 retrotransposon.

LINE-1 (L1) retrotransposition is widespread in many cancers, especially those with a high burden of chromosomal rearrangements. However, whether and to what degree L1 activity directly impacts genome integrity is unclear. Here, we apply whole-genome sequencing to experimental models of L1 expression to comprehensively define the spectrum of genomic changes caused by L1.

Gag proteins encoded by endogenous retroviruses are required for zebrafish development.

Transposable elements (TEs) make up the bulk of eukaryotic genomes and examples abound of TE-derived sequences repurposed for organismal function. The process by which TEs become coopted remains obscure because most cases involve ancient, transpositionally inactive elements. Reports of active TEs serving beneficial functions are scarce and often contentious due to difficulties in manipulating repetitive sequences.

Role of K+ and amino acids in osmoregulation by the free-living microaerophilic protozoon Hexamita inflata.

The primitive free-living protozoon Hexamita inflata was found to maintain a cell volume of approximately 260 fI under standard culture conditions. On increasing the extracellular osmolality the volume decreased and the cells remained shrunken for >30 min. By contrast, a decrease in the external osmolality resulted in a transient increase in cell volume which was followed by an efficient 'regulatory volume decrease' (RVD).