Publications by authors named "Phillip Dettleff"

The heat waves on the South Pacific coast could lead to thermal stress in native fish. The red cusk-eel (Genypterus chilensis) is relevant for Chilean artisanal fisheries and aquaculture diversification. This study examined the effect of high-temperature stress in the gills of G.

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The genes encoding for estrogen receptor () and follicle-stimulating hormone receptor () play crucial roles in ovarian follicular development. This study aimed to determine the expression levels of miRNAs predicted against and mRNAs in follicular cells related to their target genes during the estrous cycle in canines. Antral follicles were dissected from 72 ovaries following ovariohysterectomies.

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The salmonid rickettsial syndrome (SRS) is a systemic bacterial infection caused by Piscirickettsia salmonis that generates significant economic losses in Atlantic salmon (Salmo salar) aquaculture. Despite this disease's relevance, the mechanisms involved in resistance against P. salmonis infection are not entirely understood.

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Article Synopsis
  • High temperatures (19 °C) negatively impact the liver of the red cusk-eel, a species important for Chilean aquaculture, when compared to normal temperatures (14 °C).
  • Evaluations revealed significant increases in hepatic enzyme activity and oxidative stress indicators, indicating damage to liver tissues.
  • The study identified transcriptional changes with numerous genes being up-regulated or down-regulated, highlighting potential biomarkers for assessing thermal stress in aquaculture practices.
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The buoyancy of eggs and embryos is associated with successful development in pelagic fish. Buoyancy is the result of oocyte hydration, which depends on the osmotic force exerted by free amino acids (FAA) generated by yolk proteolysis, and cathepsins are the main enzymes involved in this process. is a pelagic fish whose farming has been hampered by development failure that have been partially attributed to decreased buoyancy of embryos.

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Understanding the genetic status of aquaculture strains is essential for developing management guidelines aimed at sustaining the rates of genetic gain for economically important traits, as well as securing populations that will be robust to climate change. Coho salmon was the first salmonid introduced to Chile for commercial purposes and now comprises an essential component of the country's aquaculture industry. Several events, such as admixture, genetic bottlenecks, and rapid domestication, appear to be determinants in shaping the genome of commercial strains representing this species.

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  • Salmonid rickettsial septicemia (SRS) is a major disease affecting Chile's salmon farming, with skeletal muscle notably impacted due to stress in intensive farming practices.
  • This study analyzes the RNA-seq of rainbow trout myotubes that were treated with cortisol before being infected, revealing a total of 175 genes affected, with many involved in apoptosis and immune responses.
  • Important findings suggest that cortisol pretreatment alters the trout's immune response and gene expression during infection, highlighting a link between stress hormones and pathogen effects.
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 , , and pathogenic are zoonotic pathogens that infect humans, as well as domestic and wild animals. In wild canids, they may affect their fertility and reproduction, threatening their conservation. Wild canids play a crucial role in the environment as meso- and top-predators and environmental sentinels for zoonotic pathogens.

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Skeletal muscle is the most abundant tissue in teleosts and is essential for movement and metabolism. Recently, it has been described that skeletal muscle can express and secrete immune-related molecules during pathogen infection. However, the role of this tissue during infection is poorly understood.

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In pelagic fish, embryo buoyancy is a noteworthy aspect of the reproductive strategy, and is associated with overall quality, survival, and further developmental success. In captivity, the loss of buoyancy of early embryos correlates with high mortality that might be related to massive cell death. Therefore, the aim of this study was to evaluate under captivity conditions the expression of genes related to the apoptosis process during the early embryonic development of the pelagic fish , and its relationship to the buoyancy of embryos.

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  • Shiga toxin-producing Escherichia coli (STEC) is a harmful bacteria causing serious illnesses in humans, largely linked to food contamination, and has witnessed rising antimicrobial resistance in recent years.
  • A study in Chile analyzed STEC in various food samples (meat, seafood, vegetables, and street food), finding a small percentage (0.5%) positive for STEC, with specific virulence genes and predominant resistance to colistin.
  • The research identified various sequence types (STs) of STEC, including international clones usually found in human infections, marking the first comprehensive genomic examination of these strains in Chilean food sources.
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Red cusk-eel (Genypterus chilensis) is a native species with potential for Chilean aquaculture diversification. However, no information exists on the effects of temperature on oxidative stress and eggs quality markers in post-ovulatory eggs and ovary of this species. We determine that high and low temperature generate oxidative damage on post-ovulatory eggs, with no effect on ovary.

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The red cusk-eel (Genypterus chilensis) is a native species with strong potential to support Chilean aquaculture diversification. Environmental stressors, such as temperature, may generate important effects in fish physiology with negative impact. However, no information exists on the effects of thermal stress in Genypterus species or how this stressor affects the skeletal muscle.

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  • * A study involving 771 dogs found that 7.0% tested positive for the infection, and researchers analyzed specific genomic features of the pathogen, discovering variations that could impact its virulence.
  • * The findings emphasize the importance of controlling dog populations, advocating for mandatory testing of dogs, especially strays, prior to adoption to prevent transmission to humans.
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In echinoderms, the immune system plays a relevant role in defense against infection by pathogens. Particularly, in sea urchins, the immune system has been shown to be complex, especially in terms of the variety of immune genes and molecules described. A key component of the response to external pathogens are the Toll-like receptors (TLRs), which are a well-characterized class of pattern recognition receptors (PRRs) that participate in the recognition of pathogen-associated molecular patterns (PAMPs).

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The red cusk-eel (Genypterus chilensis) is a native species with strong potential to support Chilean aquaculture diversification. Under commercial conditions, fish are exposed to several stressors. To date, little is known about the mechanism involved in the stress response of red cusk-eel, and there is no information related to the regulation mediated by long noncoding RNAs (lncRNAs).

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The red cusk-eel (Genypterus chilensis) is a native Chilean species with a high-value market, with the potential to diversify Chilean aquaculture. The objective of this study was to develop a set of microsatellite markers, estimate genetic parameters, determine population differentiation, and identify the population structure of wild and commercial populations of G. chilensis.

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  • - Growth differentiation factor 9 (GDF-9) and bone morphogenetic protein 15 (BMP-15) are crucial for the development of oocytes, and a study was conducted to investigate their roles during in vitro maturation (IVM) of canine oocytes using various treatment groups.
  • - Treatment with antibodies against GDF-9 or BMP-15 negatively affected meiotic development, resulting in a higher percentage of oocytes arrested at the germinal vesicle breakdown (GVBD) stage compared to the control group, which allowed more oocytes to progress to the metaphase I (MI) and metaphase II (MII) stages.
  • - While there was no significant difference in MII rates when comparing
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In pelagic species such as Seriola lalandi, survival of both the eggs and embryos depends on yolk processing during oocyte maturation and embryo development. The main enzymes involved in these processes are the cathepsins, which are essential for the hydration process, acquiring buoyancy and nutrition of the embryo before hatching. This study aimed to investigate the mRNA expression profiles of cathepsins B, D and L (catb, catd and catl) and the activity of these enzymes during early development in S.

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The infectious salmon anemia virus (ISAv) produces a systemic infection in salmonids, causing large losses in salmon production. However, little is known regarding the mechanisms exerting disease resistance. In this paper, we perform an RNA-seq analysis in Atlantic salmon challenged with ISAv (using individuals coming from families that were highly susceptible or highly resistant to ISAv infection).

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Article Synopsis
  • Piscirickettsia salmonis causes serious infections in salmonids, particularly affecting Atlantic salmon and leading to significant economic losses.
  • This study investigates the bacterial load in the head kidney and muscle of salmon with different survival rates, finding that resistant fish have lower bacterial loads.
  • Analysis of immune-related genes reveals that resistant fish effectively activate specific genes to reduce bacterial loads, while susceptible fish overreact without effectively combating the infection.
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Article Synopsis
  • The study focuses on the roles of growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) during the maturation of fish oocytes and early embryonic development in Seriola lalandi, a fish species important for aquaculture.
  • Results showed that GDF9 levels were highest in previtellogenic oocytes and decreased after ovulation, while BMP15 levels were also highest in these oocytes but lower than GDF9 before increasing in newly spawned eggs.
  • During embryonic development, GDF9 levels were low initially but increased after 48 hours post-fertilization, whereas BMP15 levels remained stable throughout development, indicating their distinct regulatory roles during oocyte maturation and embryo
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