Publications by authors named "Philippe T Ouakou"

The total number of Guinea worm cases has been reduced by 99.9% since the mid-1980s when the eradication campaign began. Today, the greatest number of cases is reported from Chad.

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After a ten-year absence of reported Guinea worm disease in Chad, human cases were rediscovered in 2010, and canine cases were first recorded in 2012. In response, active surveillance for Guinea worm in both humans and animals was re-initiated in 2012. As of 2018, the Chad Guinea Worm Eradication Program (CGWEP) maintains an extensive surveillance system that operates in 1,895 villages, and collects information about worms, hosts (animals and humans), and animal owners.

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Article Synopsis
  • Whole-genome sequencing is increasingly used to study helminth genomes, focusing on genome assembly, population genetics, and diagnostics, but adult parasites are difficult to access for DNA sampling.
  • The study explores five low-input DNA extraction methods along with a sequencing protocol to enable whole-genome sequencing of immature helminth stages, which typically yield low DNA quantities and face contamination issues.
  • Results showed successful sample preparations for most tested species, indicating that whole-genome sequencing of individual helminth parasites is feasible and can enhance diagnostic and surveillance efforts.
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Dracunculus medinensis, or human Guinea worm (GW), causes a painful and debilitating infection. The global Guinea Worm Eradication Program (GWEP) has successfully reduced human GW cases from 3.5 million in 21 countries in 1986 to only 30 cases in three remaining countries in 2017.

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A third-stage (infective) larva of Dracunculus medinensis, the causative agent of Guinea worm disease, was recovered from a wild-caught Phrynobatrachus francisci frog in Chad. Although green frogs (Lithobates clamitans) have been experimentally infected with D. medinensis worms, our findings prove that frogs can serve as natural paratenic hosts.

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