Assessing Phototoxicity in a Mammalian Cell Line: How Low Levels of Blue Light Affect Motility in PC3 Cells.

Phototoxicity is a significant constraint for live cell fluorescence microscopy. Excessive excitation light intensities change the homeostasis of the observed cells. Erroneous and misleading conclusions may be the problematic consequence of observing such light-induced pathophysiology.

Development of pGEMINI, a Plant Gateway Destination Vector Allowing the Simultaneous Integration of Two cDNA via a Single LR-Clonase Reaction.

Gateway technology has been used to facilitate the generation of a large number of constructs for the modification of plants for research purposes. However, many of the currently available vectors only allow the integration of a single cDNA of interest into an expression clone. The ability to over-express multiple genes in combination is essential for the study of plant development where several transcripts have a role to play in one or more metabolic processes.

The olfactory sensory map in Drosophila.

The fruit fly (Drosophila melanogaster) exhibits robust odor-evoked behaviors in response to cues from diverse host plants and pheromonal cues from other flies. Understanding how the adult olfactory system supports the perception of these odorous chemicals and translates them into appropriate attraction or avoidance behaviors is an important goal in contemporary sensory neuroscience. Recent advances in genomics and molecular neurobiology have provided an unprecedented level of detail into how the adult Drosophila olfactory system is organized.