Publications by authors named "Philippe Alain Grange"
() has been implicated in inflammatory acne where highly mutated Christie-Atkins-Munch-Petersen factor (CAMP)1 displays strong toll like receptor (TLR)-2 binding activity. Using specific antibodies, we showed that CAMP1 production was independent of phylotype and involved in the induction of inflammation. We confirmed that TLR-2 bound both mutated and non-mutated recombinant CAMP1, and peptide array analysis showed that seven peptides (A14, A15, B1, B2, B3, C1 and C3) were involved in TLR-2 binding, located on the same side of the three-dimensional structure of CAMP1.
View Article and Find Full Text PDFObjectives: We evaluated a real-time quantitative PCR (qPCR) for detection of the (TP) genome in clinical samples through simultaneous detection of two genomic targets.
Methods: We performed qPCR with TaqMan technology using two TP genes, and , as targets, with an internal positive control. The qPCR assay was compared with syphilis diagnosis based on a combination of clinical examination, serological results and inhouse nested PCR (nPCR).
is a member of the skin microbiota found predominantly in regions rich in sebaceous glands. It is involved in maintaining healthy skin and has long been considered a commensal bacterium. Its involvement in various infections has led to its emergence as an opportunist pathogen.
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- Benzathine penicillin G (BPG) is the standard treatment for early syphilis, but recent shortages have prompted the need for alternative therapies.
- A study in France assessed genetic resistance of the syphilis-causing bacteria, Treponema pallidum, to macrolides and doxycycline using swabs from 146 patients.
- Results showed a high level of macrolide resistance (85% of samples), while no resistance to doxycycline was found, suggesting doxycycline is a viable substitute for BPG in treating early syphilis.
Article Synopsis
- Treponema pallidum subsp. pallidum, the bacteria causing syphilis, was genetically analyzed in 133 clinical samples from patients in France between 2010-2016 using a Multilocus Sequence Typing system.
- The study identified 18 different allelic profiles among 112 fully typed samples, revealing notable genetic diversity, including several novel alleles.
- Results showed that patients infected with Nichols-like strains were generally older and diagnosed with secondary syphilis more frequently, with specific genetic mutations linked to antibiotic resistance present in certain allelic profiles.
Article Synopsis
- Treponema pallidum subsp. endemicum (TEN) is linked to bejel, a type of endemic syphilis, and a unique isolate (11q/j) was found in a patient returning to France from Pakistan with a syphilis-like genital lesion.
- Researchers used advanced molecular techniques to analyze this isolate, discovering it clusters with known TEN strains but shows recombination at specific genetic loci, indicating genetic mixing with other Treponema species.
- The findings highlight the importance of the 11q/j isolate in understanding TEN's evolutionary history and suggest that TEN strains may play a role in causing syphilis-like symptoms.
Background: Propionibacterium acnes (P. acnes) is an anaerobic, Gram-positive bacteria encountered in inflammatory acne lesions, particularly in the pilosebaceous follicle. P.
View Article and Find Full Text PDFBackground: The incidence of HIV-syphilis co-infection has risen since 2000, especially among men having sex with men (MSM). Syphilis reinfection can occur, but the clinical features of such events remain poorly characterized.
Objective: To compare the cutaneous lesions seen with syphilis reinfections with those of first episodes in HIV-infected patients.
Two major Treponema pallidum subtypes, 14 d/g and 14 d/f, were identified in a population of 119 patients with syphilis in Paris, France, characterized by a high proportion of men who have sex with men. A new subtype named 11 q/j was characterized, and a reinfection case was determined in 1 patient having consecuitve syphilis infection at 19-month interval.
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