Publications by authors named "Philip Neubert"
Lipids are a ubiquitous class of structurally complex molecules involved in various biological processes. In the fast-growing field of lipidomics, preanalytical issues are frequently neglected. Here, we investigated the stability of lipid profiles of murine liver, brain, lung, heart, and spleen homogenates by quantitative flow injection analysis using tandem mass spectrometry and high-resolution mass spectrometry.
View Article and Find Full Text PDFObjective: Spontaneous bacterial peritonitis (SBP) is a life-threatening complication of liver cirrhosis with a 1-year mortality of 66%. Bacterial translocation (BT) from the intestine to the mesenteric lymph nodes is crucial for the pathogenesis of SBP.
Design: Since BT presupposes a leaky intestinal epithelium, the integrity of mucus and epithelial cell junctions (E-cadherin and occludin) was examined in colonic biopsies from patients with liver cirrhosis and controls.
Article Synopsis
- The study focused on developing an enzyme-linked immunosorbent assay (ELISA) to accurately detect antibodies against SARS-CoV-2, which is crucial for monitoring the pandemic and informing public health strategies.
- The ELISA utilized specific components of the virus (receptor-binding domain and spike ectodomain) and showed high specificity (99.3%) and sensitivity for different antibody types (IgM, IgA, IgG) in patient serum samples.
- The results indicated that this test can reliably assess immune response after infection and vaccination, making it valuable for understanding protective immunity in the population.
Hippocampal surface analysis in subjective memory impairment, mild cognitive impairment and Alzheimer's dementia.
Dement Geriatr Cogn Disord
February 2009
Background/aims: Alzheimer's disease (AD) is preceded by prodromal states. To determine the neuroanatomical basis for the relationship among such states, we assessed surface conformations of the hippocampus in AD, mild cognitive impairment (MCI) and subjective memory impairment (SMI).
Methods: Hippocampal surfaces were reconstructed three-dimensionally via large-deformation high-dimensional MRI brain mapping in 14 SMI, 15 MCI, 12 AD and 13 controls.