Chimeric reporter genes were generated comprising nine different promoters of the white spot syndrome virus linked to luciferase, with the aim to compare their transcriptional activities in insect cells. The promoters included the four non-structural genes DNA polymerase, ribonucleotide reductase small subunit, ribonucleotide reductase large subunit, and thymidine-thymidylate kinase, and the five structural genes VP15, VP19, VP24, VP26, and VP28. The promoters of the non-structural but not the structural genes can function in these cells, indicating that transcription of the non-structural genes can be recognized by cellular transcriptional machineries.
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