Integration of IFAST-based nucleic acid extraction and LAMP for on-chip rapid detection of Agroathelia rolfsii in soil.

Agroathelia rolfsii (A. rolfsii) is a fungal infection and poses a significant threat to over 500 plant species worldwide. It can reduce crop yields drastically resulting in substantial economic losses.

Extraction of Heat-Stabilised Defatted Rice Bran Protein by Solid-State Fermentation Using Heterofermentative Microbes from Traditional Asian Starters.

Research Background: Heat-stabilised defatted rice bran (HSDRB) is a primary by-product of rice bran oil extraction industry and a nutritious source of protein. However, despite the unique nutritional profile of rice bran protein, the protein-rich by-product, HSDRB is underutilised as a low-value animal feed. Research on protein extraction from HSDRB by enzymatic hydrolysis has attracted the attention of numerous scientists.

Herbal species authentication by melting fingerprint coupled with high resolution melting analysis (MF-HRM).

Background: Herbal medicines have recently attracted increasing attention for use as food supplements with health benefits; however, species authentication can be difficult due to incomplete morphological characters. Here, a molecular tool was developed for the identification of species in the National List of Essential Medicinal Plants in Thailand.

Methods: The identification process used DNA fingerprints including start codon targeted (SCoT) and inter simple sequence repeat (ISSR) polymorphisms, coupled with high resolution melting (HRM), to produce melting fingerprint (MF)-HRM.

Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD).

Canine babesiosis is a tick-borne disease caused by Babesia spp., which infects and destroys healthy erythrocytes, leading to mortality and morbidity in dogs. The diagnosis of babesiosis is tedious and time-consuming, especially in latent and chronic infections.

Optimization and application of loop-mediated isothermal amplification technique for sex identification in red-whiskered bulbul ().

The red-whiskered bulbul (s) is a popular avian species in Thailand and many other countries. The red-whiskered bulbul has a high economic value, but breeding is challenging since sex identification is difficult. The PCR method is now used for sex identification.

Can feline (Felis catus) flat and long bone morphometry predict sex or skull shape?

This study demonstrates sexual dimorphism in feline bones based on morphometric analysis of dried flat bones (scapula and os coxa) and long bones (humerus, radius, ulna, femur, tibia, and fibula) of 92 felines (50 male, 42 female). A total of 58 parameters (flat bones: scapula = 4 and os coxa = 7; long bones: humerus = 8, radius = 9, ulna = 10, femur = 9, tibia = 7, and fibula = 4) were measured using a digital vernier caliper. Twenty-three parameters were found to be significantly different between cats of different sexes and skull shapes.