Papain-Catalyzed Hydrolysis of -Benzoyl-arginine--nitroanilide in an Aqueous-Organic Medium.

Enzyme-based biosensors have emerged as an effective alternative, providing simplicity, high sensitivity, and the capability to detect multiple residues. However, despite their widespread use, limited studies have examined how organic solvents inhibit these sensors. This study investigates the enzymatic reactions and structure of the selected model enzyme, papain, a protease derived from , in the presence of various organic solvents.