A Mild and Catalyst-Free Synthesis of Cannabidiols and Regioisomers Mediated by Fluorinated Alcohols.

This study presents a novel and practical strategy for synthesizing cannabinoids using a fluorinated alcohol solvent for the first time. Hexafluoroisopropanol (HFIP) was found to efficiently promote intermolecular Friedel-Crafts alkylation between allylic alcohols and electron-rich aromatics. This approach enabled the successful synthesis of cannabidiol (CBD) derivatives bearing various alkyl substituents, as well as the corresponding unnatural regioisomer (abnCBD), achieving moderate to high yields under mild conditions without the need for additional catalysts or other reagents.

Isothermal Detection of Canine Blood Parasite () Utilizing Recombinase Polymerase Amplification Coupled with Graphene Oxide Quenching-Based Pyrrolidinyl Peptide Nucleic Acid.

Canine monocytic ehrlichiosis (CME), caused by transmitted infection, is a major disease in dogs with worldwide distribution. Herein, a nucleic acid assay was established for the identification of infection employing a fluorescently labeled conformationally constrained pyrrolidinyl PNA probe (Flu-acpcPNA) designed to sequence-specifically target the 16S rRNA gene. The sensing principle is based on the excellent quenching ability of graphene oxide (GO) of the free PNA probe, that was diminished upon binding to the DNA target.