Development and validation of an LC-MSMS method for the quantitation of pacritinib; application of kinetics in rabbits.

Background: Accurate and selective LC/ESI-MSMS method development and validation for the quantitation of pacritinib is the primary goal of this study to perform kinetic studies in the healthy rabbit.

Methods: Chromatographic resolution was accomplished with a hypersil/ODS (50 mm × 4.6 mm, 3 μ) analytical C column and a mobile phase composition of 0.