The Effects of Marine Fungal Asterripeptides A-C on In Vitro and In Vivo Skin Infection.

This study aimed to investigate the in vitro and in vivo antibacterial and cytoprotective activities of marine fungal tripeptide derivatives with cinnamic acid moiety asterripeptides A-C (-). The antimicrobial and antibiofilm activities of asterripeptides A-C were tested using the ATCC 21027 strain. Human HaCaT keratinocytes infected with were used for the in vitro investigation of the various aspects of the influence of asterripeptides A-C by lumino- and fluorospectrometry, ELISA, flow cytometry, Western blotting, and microscopy techniques.

Absolute Stereochemistry and Cytotoxic Effects of Vismione E from Marine Sponge-Derived Fungus sp. 1901NT-1.2.2.

The metabolic profile of the sp. 1901NT-1.2.

Cytoprotective Polyketides from Sponge-Derived Fungus .

The new polyketides lopouzanones A and B, as well as the new 1--acetyl and 2--acetyl derivatives of dendrodochol B, were isolated from the sponge-derived marine fungus strain 168CLC-57.3. Moreover, six known polyketides, gliorosein, balticolid, dendrodolide G, dihydroisocoumarine, (-)-5-methylmellein, and dendrodochol B, were identified.

Cytotoxic Drimane-Type Sesquiterpenes from Co-Culture of the Marine-Derived Fungi KMM 4638 and (=) KMM 4639.

Chemical investigation of a coculture of the marine-derived fungi KMM 4639 and KMM 4638 led to the identification of three new drimane-type sesquiterpenes, asperflavinoids B, D and E (, , ), and nine previously reported related compounds. The structures of these compounds were established using spectroscopic methods and by comparison with known analogues. We also investigated the cytotoxic activity of the isolated compounds against several cancer and normal cell lines.

New Glycosylated Secondary Metabolites from Marine-Derived Bacteria.

Three new glycosylated secondary metabolites, including a new indole alkaloid, pityriacitrin D (), and two new trehalose lipids ( and ), together with three known compounds (-) were isolated from two marine-derived bacterial strains, 168CLC-66.1 and IV19-045. The structures of - were determined by extensive analysis and comparison of their spectroscopic data with literature values.

Genome-wide comparison deciphers lifestyle adaptation and glass biodeterioration property of Curvularia eragrostidis C52.

Glass biodeterioration by fungi has caused irreversible damage to valuable glass materials such as cultural heritages and optical devices. To date, knowledge about metabolic potential and genomic profile of biodeteriorative fungi is still scarce. Here, we report for the first time the whole genome sequence of Curvularia eragrostidis C52 that strongly degraded silica-based glasses coated with fluorine and hafnium, as expressed by the hyphal surface coverage of 46.

New Tripeptide Derivatives Asperripeptides A-C from Vietnamese Mangrove-Derived Fungus LM.5.2.

Three new tripeptide derivatives asterripeptides A-C (-) were isolated from Vietnamese mangrove-derived fungus LM.5.2.

New Polyenes from the Marine-Derived Fungus with Anti-Neuroinflammatory and Cytotoxic Activities.

Three new polyene compounds, talacyanols A-C (-), along with two known compounds, ramulosin () and eurothiocin A (), were isolated from the marine fungus derived from a seaweed sp. Structures of - were established by one-dimensional and two-dimensional (1D/2D) NMR, HR-ESIMS, and the modified Mosher's methods, as well as comparison with previously reported literature data. All the compounds (-) were tested for their in vitro cytotoxic and anti-neuroinflammatory activities.

Two New Phomaligols from the Marine-Derived Fungus and Their Anti-Neuroinflammatory Activity in BV-2 Microglial Cells.

Two new phomaligols, deketo-phomaligol A () and phomaligol E (), together with six known compounds (-) were isolated from the culture broth of the marine-derived fungus . Compound was first isolated as a phomaligol derivative possessing a five-membered ring. The structures and absolute configurations of the new phomaligols were determined by detailed analyses of mass spectrometry (MS), nuclear magnetic resonance (NMR) data, optical rotation values and electronic circular dichroism (ECD).

Neuroprotective Metabolites from Vietnamese Marine Derived Fungi of and Genera.

Low molecular weight secondary metabolites of marine fungi , and sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques.

Structure and anticancer activity of a new lectin from the cultivated red alga, Kappaphycus striatus.

The red alga Kappaphycus striatus is economically important food species and extensively cultivated throughout most tropical parts of the world as a source of carrageenan. In this note, the primary structure of a new lectin KSL from this alga was elucidated by the rapid amplification method of complementary DNA (cDNA) ends, which consists of 267 amino acid residues distributed in four tandem-repeated domains of about 67 amino acids and sharing 43% of identity. The calculated molecular mass from the deduced sequence was consistent with that of natural KSL (27,826 Da) determined by electron spray ionization-mass spectrometry.

Anti-Neuroinflammatory Agent, Restricticin B, from the Marine-Derived Fungus and Its Inhibitory Activity on the NO Production in BV-2 Microglia Cells.

A new compound containing a triene, a tetrahydropyran ring and glycine ester functionalities, restricticin B (), together with four known compounds (-) were obtained from the EtOAc extract of the marine-derived fungus . The planar structure of was determined by detailed analyses of MS, 1D and 2D NMR data. The relative and absolute configurations of were established via the analyses of NOESY spectroscopy data, the comparison of optical rotation values with those of reported restricticin derivatives and electronic circular dichroism (ECD).

Insight into Antioxidant and Photoprotective Properties of Natural Compounds from Marine Fungus.

This computational and experimental work aims to elucidate physicochemical and photophysical natures of free radical scavenging and ultraviolet radiation (UVR) filtering activities of five terpenoids available in the extract of marine fungus. The antioxidant activities of ochraceopone F (C), aspertetranone D (C), cycloechinulin (C), wasabidienone E (C), and mactanamide (C) are evaluated by using density functional theory (DFT) at the M05-2X/6-311++G(d,p) level of theory in the gas phase, water, and pentyl ethanoate (PEA). Double antioxidant mechanisms allowing the second (H/e) donation such as double hydrogen atom transfer (dHAT), double single electron transfer-proton transfer (dSET-PT), and double sequential proton loss-electron transfer (dSPL-ET) are considered.

Biologically Active Echinulin-Related Indolediketopiperazines from the Marine Sediment-Derived Fungus .

Seven known echinulin-related indolediketopiperazine alkaloids (-) were isolated from the Vietnamese sediment-derived fungus . Using chiral HPLC, the enantiomers of cryptoechinuline B () were isolated as individual compounds for the first time. (+)-Cryptoechinuline B () exhibited neuroprotective activity in 6-OHDA-, paraquat-, and rotenone-induced in vitro models of Parkinson's disease.

Resorcinosides A and B, Glycosylated Alkylresorcinols from a Marine-Derived Strain of the Fungus .

Two new glycosylated alkylresorcinols, resorcinosides A () and B (), were isolated from a strain of the fungus derived from a marine sediment sample collected from Cu Lao Cham Island, Vietnam. The structures of and were established by interpretation of 1D and 2D NMR and high-resolution ESIMS data, and their absolute configurations were confirmed by the coupling constant of the anomeric proton, acid hydrolysis, subsequent HPLC analysis, Mosher's method, and quantum-mechanics-based computational analysis of NMR chemical shifts. The structure elucidation indicated that and are new alkylresorcinols with d-glucose, and has an α-pyrone moiety attached to the aromatic ring.

Biologically Active Metabolites from the Marine Sediment-Derived Fungus .

Four new compounds were isolated from the Vietnamese marine sediment-derived fungus , one aspyrone-related polyketide aspilactonol G (), one meroterpenoid 12-epi-aspertetranone D (), two drimane derivatives (,), together with five known metabolites (,,,,,). The structures of compounds - were established by NMR and MS techniques. The absolute stereoconfigurations of compounds and were determined by a modified Mosher's method.

New Ophiobolin Derivatives from the Marine Fungus and Their Cytotoxicities against Cancer Cells.

Five new sesterterpenes, 14,15-dehydro-6--ophiobolin K (), 14,15-dehydro- ophiobolin K (), 14,15-dehydro-6--ophiobolin G (), 14,15-dehydro-ophiobolin G () and 14,15-dehydro-()-14-ophiobolin G (), together with four known ophiobolins (-) were isolated from the marine fungus derived from the seaweed sp. collected in Vietnam. The five new ophiobolins were first isolated as ophiobolin derivatives consisting of a fully unsaturated side chain.

Neuroprotective Activity of Some Marine Fungal Metabolites in the 6-Hydroxydopamin- and Paraquat-Induced Parkinson's Disease Models.

A new melatonin analogue 6-hydroxy--acetyl-β-oxotryptamine () was isolated from the marine-derived fungus sp. KMM 4672. It is the second case of melatonin-related compounds isolation from microfilamentous fungi.

Suppression of RANKL-Induced Osteoclastogenesis by the Metabolites from the Marine Fungus Aspergillus flocculosus Isolated from a Sponge Stylissa sp.

A new α-pyrone merosesquiterpenoid possessing an angular tetracyclic carbon skeleton, ochraceopone F (), and four known secondary metabolites, aspertetranone D (), cycloechinulin (), wasabidienone E (), and mactanamide (), were isolated from the marine fungus derived from a sponge sp. collected in Vietnam. The structures of Compounds - were elucidated by analysis of 1D and 2D NMR spectra and MS data.

Purification, characterization and biological effect of lectin from the marine sponge Stylissa flexibilis (Lévi, 1961).

SFL, a lectin from the marine sponge Stylissa flexibilis was purified by cold ethanol precipitation followed by ion exchange chromatography on DEAE Sepharose column and Sephacryl S-200 gel filtration. SFL is a dimeric glycoprotein of 32kDa subunits linked by a disulfide bridge with a molecular mass of 64kDa by SDS-PAGE and 65kDa by Sephacryl S-200 gel filtration. SFL preferentially agglutinated enzyme treated human A erythrocytes.