Understanding PFAS toxicity through cell culture metabolomics: Current applications and future perspectives.

Per- and polyfluoroalkyl substances (PFAS), ubiquitous environmental contaminants, pose significant challenges to ecosystems and human health. While cell cultures have emerged as new approach methodologies (NAMs) in ecotoxicity research, metabolomics is an emerging technique used to characterize the small-molecule metabolites present in cells and to understand their role in various biological processes. Integration of metabolomics with cell cultures, known as cell culture metabolomics, provides a novel and robust tool to unravel the complex molecular responses induced by PFAS exposure.

Antimicrobial resistance and biofilm formation of in a Vietnamese fish processing facility.

This study aimed to investigate the occurrence, antibiotic resistance, and biofilm formation of in the Vietnamese fish processing facility. Among 144 samples including fish, wash water, food contact surfaces, and personnel gloves, 18  isolates was detected and characterized. The was detected most frequently in wash water samples (22%, 8/36), followed by fish (18%, 8/45).

Biofilm Formation From Listeria monocytogenes Isolated From Pangasius Fish-processing Plants.

Biofilm formation of Listeria monocytogenes in food processing environments cause potential source of cross-contamination to foodstuffs; hence, the control of biofilm is currently addressed to find effective solutions for preventing biofilm formation or eliminating the established one. Forty-five strains of Listeria monocytogenes isolated from Pangasius fish-processing plants were studied for their capability to form a biofilm on 96-well microtiter plate by using the conventional crystal violet staining. Additionally, the inhibitory effect of biofilm formation by food additives including monascus pigment and ε-polylysine was examined.