Polythiophene synthesis coupled to quartz crystal microbalance and Raman spectroscopy for detecting bacteria.

A simple electrochemical procedure was used for the synthesis of a polythiophene containing para-benzenesulfonyl chloride groups. The obtained polymer was shown to be very reactive and directly able to covalently bind nucleophile biomolecules. Protein A and a specific antibody were then successively immobilized on the conductive polymer through a covalent bonding of Protein A with the as-prepared linker for bacteria trapping purpose.

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An epidemiological study has been carried on at the Amiens' Gynecology-Obstetrical center with the aim to determinate the aetiology of acute gastrointestinal disorders who affected part of the newborns over a two months period. Rotavirus was found in 16 babies (33 %) by electronmicroscopy and Enzyme-linked-Immuno-sorbent-Assay (ELISA). In one case, a « small round viruswas associated with the Rotavirus.

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A systematic survey was carried on stools from 130 children suffering of acute gastroenteritis. Electron microscopy, enzymo-linked immunosorbent assay (ELISA) and counter electrophoresis were employed. This survey allowed to the detection by electron microscopy of Rotavirus (40 cases), Coronaviruses (3 cases), Astroviruses (2 cases), Adenoviruses (2 cases) and Small Round Viruses (1 case).