Effect of Metformin on Spermatogenesis Restoration Following Spermatogonial Stem Cell Transplantation in An Azoospermia Mouse Model.

Objective: Spermatogonial stem cell transplantation (SSCT) could be a helpful strategy for fertility restoration in patients with childhood cancer. Additionally, using metformin as an antioxidant may help mitigate damage caused by chemotherapy. This study aimed to evaluate the protective effects of metformin against oxidative stress caused by busulfan and cadmiuminduced damage while examining its role in enhancing spermatogenesis restoration following SSCT.

Recovering Spermatogenesis By Protected Cryopreservation Using Metformin and Transplanting Spermatogonial Stem Cells Into Testis in an Azoospermia Mouse Model.

Cryopreservation and transplantation of spermatogonial stem cells (SSCs) may serve as a new method to restore male fertility in patients undergoing chemotherapy or radiotherapy. However, SSCs may be damaged during cryopreservation due to the production of reactive oxygen species (ROS). Therefore, different antioxidants have been used as protective agents.