[Changes in protein, lipid composition, DNA- and RNA- polymerase activity of the chromatin fraction and the nuclear matrix of the rat liver in hypovitaminosis E].

E-hypovitaminosis-induced antioxidant deficiency in rats causes changes in some properties of nuclear structures of the liver cells, i.e. fractions of transcriptionally active and repressed chromatin and nuclear matrix.

[Autoradiographic and immunohistochemical analysis of 3H-melatonin distribution in endocrine and non-endocrine organs].

The comparison of the results of the autoradiographical and immunohistochemical study showed the discrepancy between the distribution of the radioactive label and the histotopographical localization of the melatonin-immunoreactive cells in the suprarenal glands, duodenum, pancreas and spleen after five minutes, one and three hours of the 3H-melatonin injection. It was shown that the exogenous 3H-melatonin is metabolizing rapidly and the products of its metabolism are spreading all over the organism. The exogenous 3H-melatonin may be accumulating in the separate endocrine and non-endocrine cells of the different organs.

[Morphological changes in the liver in acute myocardial infarct].

The state of the liver was studied on the 3, 7 and 14 day from the beginning of infarction in 32 cases of extensive acute myocardial infarction. It is shown that in acute myocardial infarction the liver shows simultaneously dyscirculatory, destructive and compensatory-adaptative processes develop. Imbalance of histoenzymatic processes develops at early stages of acute myocardial infarction influencing the mechanisms of compensation and reparation.

[Morphologic characteristics of the conduction system in ischemic heart disease].

An autopsy study is presented of 32 cases (age: 45-80 years) with special examination of conduction system of the heart by morphological methods. The patients died of acute and repeat myocardial infarction, ischemic heart disease. The changes were manifested in association of disordered circulatory, destructive and compensatory-adaptative processes that are the background of conduction and rhythm disorders and depending on the degree of circulatory and metabolic disturbances in the contractile myocardium.

[Tocopherol-binding proteins in membranes of rat liver mitochondria].

The use of the adsorption chromatography on the hydroxyl apatite makes it possible to yield and partly purify the triton X-100-solubilized mitochondrial protein fraction of the rat liver able to bind specifically [3H]-alpha-tocopherol. The method permits removing simultaneously both free detergent and [3H]-alpha-tocopherol from the protein mixture without disturbance of the established equilibrium. When compared with methods used for the removal of free hydrophobic ligands in the in vitro binding experiments, the applied method is the most effective.

Islet cell transplantation in type I diabetes mellitus: evaluation of humoral immune response.

Four males and three females ranging in age from 20 to 35 years and afflicted with complicated Type 1-diabetes for more than 8 years underwent islet cell allotransplantation (ATx, 6 cases) and xenotransplantation (XTx, 1 case). Precultured islet cells derived from human or bovine fetal pancreata were injected into the m. rectus abdominis.

[The blood rheological indices of patients with necrobiosis lipoidica].

Rheologic parameters of the blood were examined in 17 patients with necrobiosis lipoidica before and over the course of trental (pentoxyphyllin) therapy. The study has revealed an unchanged hematocrit in the presence of significantly increased blood viscosity, red cell aggregation and stability of red cell aggregates. Another finding was a deterioration of the red cell deformability; red cell mechanical resistance was found liable to alter.

[Selective accumulation of 131I-labeled IKO-1 monoclonal antibodies in the tissues of mouse L 1210 lymphoid leukemia].

Monoclonal antibodies (MCA) ICO-1 of IgG3 isotype against 1a-like human antigens were labelled with 131I using chloramine T; a radionuclide-bound fraction was isolated by radiochromatography. Basing on the results of assessment of complement-dependent cytotoxicity against lymphocytes from the lymph nodes of BDF1 mice a conclusion was made that MCA immunological reactivity changed slightly after radionuclide tracer administration. In vitro experiments with a panel of normal and malignant cells showed 131I-MCA binding with cells of mouse lymphoid leukemia L1210 and hemocytoblastosis La as well as with spleen and lymph node normal lymphocytes.

[Prednisolone sensitivity of peripheral blood mononuclear cells of patients with systemic lupus erythematosus clinically resistant to glucocorticoids and the effects of carbon hemoperfusion].

A study was made of the sensitivity to prednisolone (PS) of peripheral blood mononuclear cells (MS) of patients with systemic lupus erythematosus (SLE) with renal lesion showing resistance to glucocorticoids (GC). It was shown that in most of the patients (60%) MC were poorly inhibited by PS whereas the rest of the patients (40%) demonstrated in vitro sensitivity compared to that of the donors and even higher in some cases. Thus, the group of SLE patients showing clinical resistance to GC action was inhomogeneous in sensitivity of their MC to PS; only some patients showed resistance associated with the properties of the MC system.

[Accumulation of 125J-triiodothyronine in the transplanted tumors of mice and rats].

Investigations carried out in various transplantable tumours (Lewis carcinoma, carcinoma-37, melanoma B-16 in mice and sarcoma-45 in rats) did not show 125J-triiodothyronine accumulation by the tumour.