The integration of omics and cultivation-dependent methods could effectively determine the biological risks associated with the utilization of soil conditioners in agriculture.

In the circular economy, reusing agricultural residues, treated biowaste, and sewage sludges-commonly referred to as soil conditioners-in agriculture is essential for converting waste into valuable resources. However, these materials can also contribute to the spread of antimicrobial-resistant pathogens in treated soils. In this study, we analyzed different soil conditioners categorized into five groups: compost from source-separated biowaste and green waste, agro-industrial digestate, digestate from anaerobic digestion of source-separated biowaste, compost from biowaste digestate, and sludges from wastewater treatment plants.

A Snapshot, Using a Multi-Omic Approach, of the Metabolic Cross-Talk and the Dynamics of the Resident Microbiota in Ripening Cheese Inoculated with .

Raw milk cheeses harbor complex microbial communities. Some of these microorganisms are technologically essential, but undesirable microorganisms can also be present. While most of the microbial dynamics and cross-talking studies involving interaction between food-derived bacteria have been carried out on agar plates in laboratory-controlled conditions, the present study evaluated the modulation of the resident microbiota and the changes of metabolite production directly in ripening raw milk cheese inoculated with strains.

Effect of Hot-Pressing Temperature on the Properties of Eco-Friendly Fiberboard Panels Bonded with Hydrolysis Lignin and Phenol-Formaldehyde Resin.

Lignin is the natural binder in wood and lignocellulosic plants and is regarded as the main natural and renewable source of phenolic compounds. Its incorporation in the composition of fiberboards will enhance both the environmental performance of the panels and the complex use of natural resources. In recent years, the increased valorization of hydrolysis lignin in value-added applications, including adhesives for bonding fiberboard panels, has gained significant research interest.

Uncommon Salmonella Infantis Variants with Incomplete Antigenic Formula in the Poultry Food Chain, Italy.

Uncommon Salmonella Infantis variants displaying only flagellar antigens phenotypically showed identical incomplete antigenic formula but differed by molecular serotyping. Although most formed rough colonies, all shared antimicrobial resistances and the presence of usg gene with wild-type Salmonella Infantis. Moreover, they were undistinguishable wild-type Salmonella Infantis by whole-genome sequencing.

Synthesis, Hydrolytic Stability and In Vivo Biological Study of Bioconjugates of the Tetrapeptides FELL Containing Pyrrole Moiety.

Background: Bioconjugates are promising alternatives for the multiple targeting of any disease. Pyrrole heterocycle is well known with many activities and is a building block of a lot of medical drugs. On the other hand, peptides are short molecules with many advantages such as small size, ability to penetrate the cell membrane and bond-specific receptors, vectorizing potential, etc.

Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization in spp. isolates from Italy reveal high heterogeneity among serovars.

Introduction: The spread of antimicrobial resistance among zoonotic pathogens such as is a serious health threat, and mobile genetic elements (MGEs) carrying antimicrobial resistance genes favor this phenomenon. In this work, phenotypic antimicrobial resistance to commonly used antimicrobials was studied, and the antimicrobial resistance genes (ARGs) and plasmid replicons associated with the resistances were determined.

Methods: Eighty-eight Italian strains ( = 88), from human, animal and food sources, isolated between 2009 and 2019, were selected to represent serovars with different frequency of isolation in human cases of salmonellosis.

Synthesis, In Silico Log Study, and In Vitro Analgesic Activity of Analogs of Tetrapeptide FELL.

Background: The inflammatory process represents a specific response of the organism's immune system. More often, it is related to the rising pain in the affected area. Independently of its origin, pain represents a complex and multidimensional acute or chronic subjective unpleasant perception.

Synthesis, Antiproliferative Effect and In Silico LogP Prediction of BIM-23052 Analogs Containing Tyr Instead of Phe.

(1) Background: Hydrophobicity (or lipophilicity) is a limiting factor in the ability of molecules to pass through cell membranes and to perform their function. The ability to efficiently access cytosol is especially important when a synthetic compound has the potential to become a drug substance. D-Phe-Phe-Phe-D-Trp-Lys-Thr-Phe-Thr-NH (BIM-23052) is a linear analog of somatostatin with established in vitro GH-inhibitory activity in nanomolar (nm) concentrations and high affinity to different somatostatin receptors.

Whole-Genome Sequence of Salmonella enterica Serovar Bispebjerg from Turkey Reveals Its Pathogenic Potential.

We report the genome sequence of a Salmonella enterica subsp. serovar Bispebjerg strain that was isolated from a turkey flock in 2011. The genome analysis of the strain, a rare and multihost serovar, revealed its pathogenic potential due to antimicrobial resistance and a plethora of Salmonella pathogenicity islands and virulence factors.

Colonization Slightly Affects Gut Microbiota and Hematological Parameters in Clinically Healthy Dogs.

() is a worldwide cause of acute diarrheal disease both in humans and animals. The primary aim of this study was to investigate possible variations in gut microbiota in a population of asymptomatic dogs ( = 31), naturally infected or not by . Gut microbiota and the hematological, biochemical, and fecal parameters related to intestinal function were investigated.

Large-scale impact of the 2016 Marine Heatwave on the plankton-associated microbial communities of the Great Barrier Reef (Australia).

The Great Barrier Reef (GBR) is the world's largest coral ecosystem and is threatened by climate change. This study investigated the impact of the 2016 Marine Heatwave (MHW) on plankton associated microbial communities along a ∼800 km transect in the GBR. 16S rRNA gene metabarcoding of archived plankton samples collected from November 2014 to August 2016 in this region showed a significant increase in Planctomycetes and bacteria belonging to the genus Vibrio and Synechococcus during and after the heatwave.

Anthroponotic-Based Transfer of to Dog: A Case Study.

Although usually harmless, spp. can cause nosocomial and community-onset skin and soft tissue infections in both humans and animals; thus, it is considered a significant burden for healthcare systems worldwide. Companion animals have been identified as potential reservoirs of pathogenic with specific reference to Methicillin Resistant (MRSA).

Effect of pH and Salinity on the Ability of Serotypes to Form Biofilm.

is a major cause of food-borne infections in Europe, and the majority of human infections are caused by only a few serotypes, among them are subsp. serotype Enteritidis (hereafter Enteritidis), Typhimurium, and the monophasic variant of . Typhimurium.

Identification and characterization of a spreadable IncI1 plasmid harbouring a bla gene in an Italian human isolate of Salmonella serovar Napoli.

Salmonella enterica subsp. enterica serovar Napoli (S. Napoli) ranks among the top serovars causing human infections in Italy, although not common in other European countries.

The Interplay between Campylobacter and the Caecal Microbial Community of Commercial Broiler Chickens over Time.

is the most frequent foodborne zoonotic bacteria worldwide, with chicken meat being overwhelmingly the most important reservoir for human infections. Control measures implemented at the farm level (i.e.

Effect of sterilization on the canine vaginal microbiota: a pilot study.

Background: Surgical sterilization is the most effective method of contraception for dogs. It also prevents pyometra and reduces the risk of mammary tumour development. However, this procedure also has negative effects, such as urinary incontinence.

Whole-genome characterisation of TEM-1 and CMY-2 β-lactamase-producing Salmonella Kentucky ST198 in Lebanese broiler chain.

Objectives: Salmonella enterica subsp. enterica serovar Kentucky has been associated with the worldwide ciprofloxacin-resistant (CIP) Salmonella Kentucky sequence type 198 (ST198) epidemic clone, mostly recovered from poultry farms and products. The aim of this study was to examine whether this expanding clone exists in the Lebanese broiler chain.

Genome Sequence of a Persistent Campylobacter jejuni Strain, 2016-IZSVE-19-111250.

In this report, we present the whole-genome sequence of a strain isolated recursively for the last 3 years from an Italian poultry farm.

Insight into an outbreak of Salmonella Choleraesuis var. Kunzendorf in wild boars.

An unusual mortality of wild boars occurred in Italy from 2012 to 2015 due to Salmonella Choleraesuis infection. In order to confirm the occurrence of an outbreak of S. Choleraesuis in wild boars and to epidemically characterise the unique S.

Characterizing Serovar Choleraesuis, var. Kunzendorf: A Comparative Case Study.

Different serovars generally display different antigenic , but there are some exceptions. For instance, the same antigenic , 6,7:c:1,5, is shared by serovar, Paratyphi C, Typhisuis, and Choleraesuis. Moreover, three biotypes have been described within the .

Evaluation and quantification of antimicrobial residues and antimicrobial resistance genes in two Italian swine farms.

Antimicrobial resistance genes (ARGs) are considered emerging environmental pollutants, posing potential risks for human and animal health: the misuse of antimicrobials in food-producing animals could favour the maintenance and spread of resistances in bacteria. The occurrence of ARGs in Italian swine farming - which has specific characteristics - was investigated in order to explore resistance spread dynamics. Two farrow-to-finish pig farms were longitudinally monitored: faecal samples from animals and environmental samples were collected.

Different Resolution Power of Multilocus Variable-Number Tandem Repeat Analysis and Whole-Genome Sequencing in the Characterization of 1,4,[5],12:i:- Isolates.

serovar 1,4,[5],12:i:- has emerged over the last two decades as one of the most common serovars causing human salmonellosis in Europe. It is supposed to originate from serovar Typhimurium due to antigenic and genotypic similarities between the two serovars. Due to the high level of similarity, the multilocus variable-number tandem repeat analysis (MLVA) protocol designed for Typhimurium routine typing is commonly used also for the characterization of 1,4,[5],12:i.