Interferon-alpha-induced gene expression: evidence for a selective effect of ouabain on activation of the ISGF3 transcription complex.

Binding of interferons (IFNs) to their cell surface receptors stimulates rapid translocation of cytoplasmic proteins to the nucleus and the expression of a variety of cellular genes within minutes. Translocated proteins subsequently bind to the interferon-stimulated response element (ISRE) located in the promoters of all IFN-activated cellular genes. We report here that ouabain, a specific inhibitor of the Na/K ATPase, selectively inhibited transcription of several IFN-alpha-induced cellular RNAs under conditions in which some other well-described signal transduction pathways remained intact.

Analysis of the lsi region involved in lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.

The genetic locus (lsi-1) responsible for the transformation of the lipooligosaccharide (LOS)-defective Neisseria gonorrhoeae mutant FA5100 to LOS expression was studied by deletion mutagenesis and sequence analysis. An open reading frame that was preceded by a leader sequence containing regions with the potential to form hairpin loops was identified. A perfect sigma 70 promoter consensus sequence was found upstream from this open reading frame.

Molecular analysis of lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.

A HindIII gene bank of Neisseria gonorrhoeae MUG116 was constructed in the cosmid vector pHC79. A cosmid (pSY81) was isolated that was able to convert N. gonorrhoeae FA5100 to reactivity with monoclonal antibody (MAb) 2-1-L8.

Use of transformation to construct Neisseria gonorrhoeae strains with altered lipooligosaccharides.

DNA isolated from a nalidixic acid- and rifampin-resistant derivative of Neisseria gonorrhoeae serum-resistant strain 302 (MUG116), a strain that reacts with monoclonal antibody (MAb) 2-1-L8, was used to transform N. gonorrhoeae DOV, a serum-sensitive strain, to antibiotic resistance and/or reactivity with the MAb. MAb 2-1-L8 binds to a 3.