Soybean molasses can be used as a substitute for corn in grazing beef cattle supplements during the rainy season.

Soybean molasses (SBMO) is a byproduct derived from the production of soy protein concentrate, obtained through solubilization in water and alcohol. The utilization of SBMO as an animal feed ingredient shows promising potential, primarily due to its low cost and as a potential energy concentrate. This study aimed to assess the intake, digestibility, ruminal parameters (pH and ruminal ammonia - NH), nitrogen retention (NR) and microbial protein synthesis in grazing beef cattle supplemented with SBMO as a substitute for corn during the rainy season.

PVRIG is Expressed on Stem-Like T Cells in Dendritic Cell-Rich Niches in Tumors and Its Blockade May Induce Immune Infiltration in Non-Inflamed Tumors.

Cancers that are poorly immune infiltrated pose a substantial challenge, with current immunotherapies yielding limited clinical success. Stem-like memory T cells (TSCM) have been identified as a subgroup of T cells that possess strong proliferative capacity and that can expand and differentiate following interactions with dendritic cells (DCs). In this study, we explored the pattern of expression of a recently discovered inhibitory receptor poliovirus receptor-related immunoglobulin domain protein (PVRIG) and its ligand, poliovirus receptor-related ligand 2 (PVRL2), in the human tumor microenvironment.

Nutritional Performance of Grazing Beef Cattle Supplemented with High-Protein Distillers' Dried Grain.

The objective was to evaluate the effects of including high-protein distillers dried grains (HP-DDG; 430 g/CP) in supplements for beef cattle in an intensive finishing pasture system. Five Nellore bulls with an average body weight (BW) of 413.5 ± 32 kg were distributed in a 5 × 5 Latin square design.

[Interhemispheric asymmetry of EEG rhythm connections during spontaneous awakenings after short sleep episodes during a monotonous psychomotor test].

Objective: To study interhemispheric asymmetry (IHA) according to electroencephalography (EEG) data of a healthy person during cognitive awakening from the second stage of daytime sleep before restoring the performance of the psychomotor test.

Material And Methods: In 23 healthy adult subjects, we studied IHA in the amplitude-amplitude interaction of EEG rhythms for 20 sec segments before spontaneous awakening determined by the moment the alpha-rhythm appearance on the EEG and the subsequent onset of psychomotor activity. The state of the subject during this period (in the initial stage of the so-called cognitive awakening preceding the behavioral awakening), when the person is unable to move, but is able to perceive external stimuli, is an experimental model for highlighting signs of conscious activity of patients when coming out of a coma.

[Morphological substantiation of laparoscopic suturing of stomach ulcer with formation of a covered perforation].

Objective: Morphological substantiation of laparoscopic suturing of gastric ulcer with formation of a covered perforation.

Material And Methods: To analyze morphological tissue reaction in surgical area, we used 12 Chinchilla rabbits. All animals were divided into 2 groups by 6 animals.

[Venous thromboembolic complications in patients with intracerebral hemorrhage].

Objective: To evaluate the effectiveness and safety of various heparin therapy regimens for venous thromboembolic complications in patients with acute cerebral circulatory disorders of the hemorrhagic type.

Material And Methods: In a prospective single-center study, treatment results of 62 patients with hypertensive brain hematoma were analyzed. All patients were divided into two comparable groups: the group of «very early» prophylactic heparin therapy or the first 48 hours from the moment of the disease (=35) and the group of «early» prophylactic heparin therapy, or later than 48 hours from the moment of the intracerebral hematoma development (=27).

Comparison of transcriptional initiation by RNA polymerase II across eukaryotic species.

The preinitiation complex (PIC) for transcriptional initiation by RNA polymerase (Pol) II is composed of general transcription factors that are highly conserved. However, analysis of ChIP-seq datasets reveals kinetic and compositional differences in the transcriptional initiation process among eukaryotic species. In yeast, Mediator associates strongly with activator proteins bound to enhancers, but it transiently associates with promoters in a form that lacks the kinase module.

[Sleep and neurophysiological correlates of consciousness activation upon awakening].

The authors discuss modern ideas about the neurophysiological mechanisms of awakening from sleep and the results of own EEG studies of the spatio-temporal dynamics of the activity of the cerebral hemispheres using the own experimental model for studying consciousness in the sleep-wake paradigm. This model is based on continuous execution of a monotonous psychomotor test performed lying down with eyes closed and allows observing several short-term sleep episodes during a 1-hour experiment, followed by spontaneous awakening and restoration of the psychomotor test. A necessary condition for the restoration of activity during spontaneous awakening is the emergence of the EEG alpha rhythm, the parameters of which determine the effectiveness of the restoration of the psychomotor test and, accordingly, the achievement of a certain level of consciousness, and therefore can be considered as a neurophysiological correlate of consciousness activation upon awakening.

Preterm premature rupture of membranes: prediction of risks in women of Zaporizhzhia region of Ukraine.

Unlabelled: The etiology of preterm premature rupture of membranes (PPROM), which is responsible for approximately 30% cases of preterm birth (PTB) is not yet fully understood.

Aim: The aim of the study was to create a mathematical model for prognostication of PPROM based on the anamnesis, clinical data, laboratory findings and genetics predictors.

Materials And Methods: The study involved 80 women with PPROM (between 26 and 34 weeks of gestation) and 50 women having term birth (>37 weeks of gestation) of Zaporizhzhia region of Ukraine.

USE OF ANTHROPOMORPHIC HETEROGENEOUS PHYSICAL PHANTOMS FOR VALIDATION OF COMPUTATIONAL DOSIMETRY OF MEDICAL PERSONNEL AND PATIENTS.

In the dosimetry of ionizing radiation, the phantoms of the human body, which are used as a replacement for thehuman body in physical measurements and calculations, play an important, but sometimes underestimated, role.There are physical phantoms used directly for measurements, and mathematical phantoms for computationaldosimetry. Their complexity varies from simple geometry applied for calibration purposes up to very complex, whichsimulates in detail the shapes of organs and tissues of the human body.

Prevention of perinatal complications in pregnant women with chronic papillomavirus infection.

Unlabelled: Social trends, a new style of modern young woman, which includes the polygamy of sexual relationships, increases the incidence and probability of infection caused by sexually transmitted pathogens, including the human papillomavirus. In women entered into motherhood with chronic papillomavirus carrier, pregnancy is accompanied with an increase in obstetrics and perinatal complications. The inability to use antiviral therapy due to its embryo- and fetotoxic effects requires searching for safe agents for timely and effective preconceptional and prenatal preparation for the fetal infection prevention.

Transcriptional Patterning of the Ventricular Cardiac Conduction System.

Rationale: The heartbeat is organized by the cardiac conduction system (CCS), a specialized network of cardiomyocytes. Patterning of the CCS into atrial node versus ventricular conduction system (VCS) components with distinct physiology is essential for the normal heartbeat. Distinct node versus VCS physiology has been recognized for more than a century, but the molecular basis of this regional patterning is not well understood.

A Critical Role for Estrogen-Related Receptor Signaling in Cardiac Maturation.

Rationale: The heart undergoes dramatic developmental changes during the prenatal to postnatal transition, including maturation of cardiac myocyte energy metabolic and contractile machinery. Delineation of the mechanisms involved in cardiac postnatal development could provide new insight into the fetal shifts that occur in the diseased heart and unveil strategies for driving maturation of stem cell-derived cardiac myocytes.

Objective: To delineate transcriptional drivers of cardiac maturation.

Direct Comparison of Mononucleated and Binucleated Cardiomyocytes Reveals Molecular Mechanisms Underlying Distinct Proliferative Competencies.

The mammalian heart is incapable of regenerating a sufficient number of cardiomyocytes to ameliorate the loss of contractile muscle after acute myocardial injury. Several reports have demonstrated that mononucleated cardiomyocytes are more responsive than are binucleated cardiomyocytes to pro-proliferative stimuli. We have developed a strategy to isolate and characterize highly enriched populations of mononucleated and binucleated cardiomyocytes at various times of development.

Requirements for RNA polymerase II preinitiation complex formation in vivo.

Transcription by RNA polymerase II requires assembly of a preinitiation complex (PIC) composed of general transcription factors (GTFs) bound at the promoter. In vitro, some GTFs are essential for transcription, whereas others are not required under certain conditions. PICs are stable in the absence of nucleotide triphosphates, and subsets of GTFs can form partial PICs.

Neurophysiological markers of high anxiety level in man during the process of preparing for a visual recognition.

By means of EEG analysis the functional state of subjects with high and low levels of anxiety was studied in different periods preceding a cognitive task - a visual expression recognition. Several conditions were investigated: background/eyes closed; background/eyes opened; listening the instruction for the cognitive task; operative rest (time lapse between listening the instruction and the beginning of the task), as well as short intervals immediately preceding the exposition of target stimuli (stage of preparation) - pairs of faces pictures with identical or different emotional expressions. At all these pre-task stages high-anxiety subjects exhibited much lower amplitude values in alpha and theta bands (as compared with low-anxiety subjects).

Evidence that Mediator is essential for Pol II transcription, but is not a required component of the preinitiation complex in vivo.

The Mediator complex has been described as a general transcription factor, but it is unclear if it is essential for Pol II transcription and/or is a required component of the preinitiation complex (PIC) in vivo. Here, we show that depletion of individual subunits, even those essential for cell growth, causes a general but only modest decrease in transcription. In contrast, simultaneous depletion of all Mediator modules causes a drastic decrease in transcription.

Mediator Undergoes a Compositional Change during Transcriptional Activation.

Mediator is a transcriptional co-activator recruited to enhancers by DNA-binding activators, and it also interacts with RNA polymerase (Pol) II as part of the preinitiation complex (PIC). We demonstrate that a single Mediator complex associates with the enhancer and core promoter in vivo, indicating that it can physically bridge these transcriptional elements. However, the Mediator kinase module associates strongly with the enhancer, but not with the core promoter, and it dissociates from the enhancer upon depletion of the TFIIH kinase.

[Pathomorphological characteristics and risk factors of asymptomatic cerebral infarction based on the results of a cross-sectional hospital-based study].

Aim: To study the prevalence, pathomorphological characteristics and risk factors of asymptomatic cerebral infarction (CI) based on the results of post-mortem studies and clinical charts of patients.

Material And Methods: The autopsies of 735 patients died due to different diseases were analyzed using continuous sampling method. CI lesions were found in 86 patients (47 women, 39 men, mean age 76.

THE DETERMINATION OF CORRELATION LINKAGES BETWEEN LEVEL OF REACTIVE OXYGEN SPECIES, CONTENTS OF NEUTROPHILES AND BLOOD GAS COMPOSITION IN EXPERIMENTAL ACUTE LUNG INJURY.

Acute lung injury (ALI) remains a major cause of acute respiratory failure and death of patients. Despite the achievements at the current stage in treatment, morbidity and mortality of ALI remain high. However, a deeper understanding of the pathogenetic links of ALI, identifying of the predictors that positively or negatively influence on the course of the syndrome, the correlation between some pathogenetic mechanisms will improve therapeutic strategies for patients with ALI, which makes the actuality of this study.

[GENUS ANELLOVIRIDAE VIRUSES IN CHRONIC LIVER DISEASE].

Aim: Viruses from genus Anelloviridae (TTV, TTMDV and TTMV) are small DNA viruses that are widespread in human popu- lation. Data on tissue tropism, cell localization and morphometry of anelloviruses are scarce. The purpose of this study was to determine the prevalence of TTV, TTMDV and TTMV in persons with liver disease and in healthy individuals, as well as electron-microscopic verification of Anelloviridae species.

[Relationships between the Flexibility of Cognitive Performance and the Response of Alpha-Rhythm in the Go/NoGo Task].

During the experiments, healthy subjects (n = 35) completed Go/NoGo task with a set towards the recognition of negative face expression; the visual stimuli were set in the middle of a 16-second-long interval between target (face) and triggering stimuli. The local changes in low-frequency alpha-oscillations in response to stimuli (desynchronization after a positive Go stimulus and synchronization after an inhibitory NoGo stimulus) take place in posterior frontal and anterior temporal lobes of the left hemisphere, i.e.

[Brain Organization of the Preparation for Visual Recognition in Preadolescent Children].

The brain organization of the process of preparation for the perception of incomplete images fragmented to different extents. The functional connections of ventrolateral and dorsoventral cortical zones with other zones in 10-11-year-old and 11-12-year-old children were studied at three successive stages of the preparation for the perception of incomplete images. These data were compared with those obtained for adults.