Publications by authors named "Petra van Leenen"
CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation of DNA double-strand breaks (DSBs) to stimulate homology-directed repair (HDR) or non-homologous end joining (NHEJ)-mediated integration of the desired knock-in. A major downside associated with DSB-mediated genome editing is the required clonal selection and expansion of candidate organoids to verify the genomic integrity of the targeted locus and to confirm the absence of off-target indels.
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- The study focuses on how genetic diversity in tumors arises, particularly the formation and spread of karyotype alterations in human tumors at a single-cell level.
- Researchers developed a method called 3D Live-Seq that combines live imaging of tumor organoids and whole-genome sequencing to track changes in tumor cells over generations.
- Findings show that karyotype changes occur rapidly and can either happen gradually through multiple cell divisions or emerge suddenly from a single division, highlighting the complex evolution of tumor genomes.