RNA Characterization in Trichoderma reesei.

This chapter provides an overview on different methods for the characterization of RNAs in Trichoderma reesei. In the first section, protocols for the extraction of total RNA from fungal mycelia and the identification of 5' and 3' ends of certain RNAs of interest via rapid amplification of cDNA ends (RACE) are presented. In the next section, this knowledge on the transcriptional start and end points is used for in vitro synthesis and fluorescence labeling of the RNA of interest.

Regulatory systems for gene expression control in cyanobacteria.

As photosynthetic microbes, cyanobacteria are attractive hosts for the production of high-value molecules from CO and light. Strategies for genetic engineering and tightly controlled gene expression are essential for the biotechnological application of these organisms. Numerous heterologous or native promoter systems were used for constitutive and inducible expression, yet many of them suffer either from leakiness or from a low expression output.

Regulation of gene expression by the action of a fungal lncRNA on a transactivator.

Long non-coding RNAs (lncRNAs) are crucial factors acting on regulatory processes in eukaryotes. Recently, for the first time in a filamentous fungus, the lncRNA was characterized in the ascomycete . In industry, this fungus is widely applied for the high-yield production of cellulases.

A current view on long noncoding RNAs in yeast and filamentous fungi.

Long noncoding RNAs (lncRNAs) are crucial players in epigenetic regulation. They were initially discovered in human, yet they emerged as common factors involved in a number of central cellular processes in several eukaryotes. For example, in the past decade, research on lncRNAs in yeast has steadily increased.

A long noncoding RNA promotes cellulase expression in .

Background: Due to its capability to secrete large quantities of plant biomass degrading enzymes (PBDE), is widely applied for industrial purposes. In nature, expression of PBDE is efficiently regulated in this fungus. Several factors involved in this regulatory network have been identified.