Exocrine gland-resident memory CD8 T cells use mechanosensing for tissue surveillance.

Tissue-resident CD8 T cells (T) continuously scan peptide-MHC (pMHC) complexes in their organ of residence to intercept microbial invaders. Recent data showed that T lodged in exocrine glands scan tissue in the absence of any chemoattractant or adhesion receptor signaling, thus bypassing the requirement for canonical migration-promoting factors. The signals eliciting this noncanonical motility and its relevance for organ surveillance have remained unknown.

Naïve Primary Mouse CD8 T Cells Retain Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering.

CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8 T cell biology, it remains elusive to what extent CRISPR/Cas9 affects function of CD8 T cells. Here, we optimized nucleofection-based CRISPR/Cas9 genetic engineering of naïve and -activated primary mouse CD8 T cells and tested their immune responses.