Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic spp. in Wild Rodents.

Giardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs for but are predominantly populated by the two rodent species and . Currently, the detection of zoonotic and non-zoonotic species and genotypes in these animals relies on cumbersome PCR and sequencing approaches of genetic marker genes.

Suitability of current typing procedures to identify epidemiologically linked human Giardia duodenalis isolates.

Background: Giardia duodenalis is a leading cause of gastroenteritis worldwide. Humans are mainly infected by two different subtypes, i.e.