Element contents and their seasonal dynamics in leaves of alder Alnus glutinosa (L.) Gaertn.

Alnus glutinosa is an actinorhizal plant that fixes N via actinomycetes. Compared to other trees, A. glutinosa is more resistant to environmental stress and able to uptake soil nutrients more easily.

Carbohydrate analysis: from sample preparation to HPLC on different stationary phases coupled with evaporative light-scattering detection.

After 20 years of development, evaporative light-scattering detection (ELSD) has become the mainstream choice for the detection of various classes of natural products. ELSD continues to grow in popularity as a "quasi-universal" technique because of the specificity of the detection method, which is based on the scattering of laser light from nonvolatile analyte particles. It represents an attractive alternative compared to other types of detection, such as refractive index detection and/or ultraviolet detection.

Supercritical fluid extraction of amino acids from birch (Betula pendula Roth) leaves and their liquid chromatographic determination with fluorimetric detection.

A method for supercritical fluid extraction (SFE) of amino acids was adapted and optimal experimental conditions were selected for a matrix consisting of dry leaves. The matrix-dependent SFE method uses a mixture of MeOH-H(2)O-acetonitrile (10:10:1 v/v/v) as a modifier (0.5 mL in situ, 300 muL on-line) at 70 degrees C and 40 MPa and no HCl is needed as an entrainer.