Erratum to: Prospects for the Use of Marine Sulfated Fucose-Rich Polysaccharides in Treatment and Prevention of COVID-19 and Post-COVID-19 Syndrome.

[This corrects the article DOI: 10.1134/S1068162022060152.].

Prospects for the Use of Marine Sulfated Fucose-Rich Polysaccharides in Treatment and Prevention of COVID-19 and Post-COVID-19 Syndrome.

Symptoms of the new coronavirus infection that appeared in 2019 (COVID-19) range from low fever and fatigue to acute pneumonia and multiple organ failure. The clinical picture of COVID-19 is heterogeneous and involves most physiological systems; therefore, drugs with a wide spectrum of mechanism of action are required. The choice of the treatment strategy for post-COVID-19 syndrome is still a challenge to be resolved.

Exosomal and non-exosomal miRNA expression levels in patients with HCV-related cirrhosis and liver cancer.

Patients with HCV-related cirrhosis are at risk for liver cancer development. For these patients miRNAs may serve as preclinical markers, which expression levels are deregulated in cancer and which are stable to the damaging factors partly through complex formation with proteins or packaging into exosomes. In this research we have tried to identify what miRNA fraction in plasma - exosomal or not packed into exosomes (non-exosomal) - is stronger associated with primary liver cancer.

Striking up with the in crowd: When option markets and insiders agree.

We study whether the trading behavior of corporate insiders provides additional information to the market, after controlling for the public information integrated by sophisticated investors. First, we establish that insiders and option market participants trade in the same direction on average. Second, we show that insidertrading is relatively more informed when the option market sentiment is positive.

[Dynamics of viral hepatitis A morbidity in Minsk].

The results of the analysis of hepatitis A morbidity during the period of 1996 - 2001, are presented. The cyclic character of this morbidity in its dynamics over the period of several years was noted with the maximum morbidity level reached in 2000. The monthly dynamics of hepatitis A morbidity reflected its seasonal character with the maximum increase in autumn and winter.

Zidampidine, an aryl phosphate derivative of AZT: in vivo pharmacokinetics, metabolism, toxicity, and anti-viral efficacy against hemorrhagic fever caused by Lassa virus.

The pharmacokinetics, metabolism, and toxicity of Zidampidine, an aryl phosphate derivative of AZT, 3'-azidothymidine-5'-[p-bromophenyl methoxyalaninyl phosphate] were investigated in CD-1 mice. Following iv injection, Zidampidine was rapidly converted to its metabolites Ala-AZT-MP and AZT. Zidampidine was not toxic to mice at doses up to 250mg/kg.

CNS activity of Pokeweed anti-viral protein (PAP) in mice infected with lymphocytic choriomeningitis virus (LCMV).

Background: Others and we have previously described the potent in vivo and in vitro activity of the broad-spectrum antiviral agent PAP (Pokeweed antiviral protein) against a wide range of viruses. The purpose of the present study was to further elucidate the anti-viral spectrum of PAP by examining its effects on the survival of mice challenged with lymphocytic choriomeningitis virus (LCMV).

Methods: We examined the therapeutic effect of PAP in CBA mice inoculated with intracerebral injections of the WE54 strain of LCMV at a 1000 PFU dose level that is lethal to 100% of mice within 7-9 days.

Stampidine prevents mortality in an experimental mouse model of viral hemorrhagic fever caused by lassa virus.

Background: The potential use of microorganisms as agents of biological warfare (BW) is a growing concern. Lassa virus, a member of the Arenavirus class of Hemorrhagic fever (HF) viruses has emerged as a worldwide concern among public health officials. The purpose of the present study was to further elucidate the antiviral activity spectrum of stampidine, a novel nucleoside analog with potent anti-viral activity against the immunodeficiency viruses HIV-1, HIV-2, and FIV, by examining its effects on survival of mice challenged with Lassa virus.

[The isolation and results of the study of the protein structure of spirochetes isolated from I. ricinus ticks in Byelarus].

Four hundred and eighty Ixodes ricinus (278 female and 202 male) samples collected in the natural biotopes of 13 administrative districts in southwest Byelarus were studied via DSK-H medium (Sigma) inoculation. Twenty-four spirochetes isolates (3, 2, 1, and 18 in the Brest, Gomel, Mogilev, and Grodno regions, respectively) were obtained on the territory of Byelarus. After adapted to the medium, most isolates (as many as 7 x 10(6)-5 x 10(7) microbial cells per ml) in the stationary phase.

[False-positive reactions in laboratory diagnosis of Lassa, Marburg, and Ebola viral hemorrhagic fevers and AIDS].

Sera of normal subjects and AIDS patients living in Minsk and Odessa were tested for antibodies to hazardous viral infections Lassa, Marburg, and Ebola. Four to 16% of examinees were seropositive to Ebola virus, 0.8 to 2.

[The cultivation and physicochemical properties of the Josiah strain of the Lassa virus].

Data on the stability of Lassa virus, Josia strain, isolated from man to the effect of physicochemical factors (heating at 50 degrees C, solutions of urea and formalin of various concentrations, UV irradiation) as well as on the time course of this strain reproduction in cell lines of different origins are presented. Recommendations for lowering of reactogenicity of the virus-containing material are given. The experimental results must be taken into consideration in the development and manufacture of diagnostic and therapeutic-prophylactic preparations for Lassa fever.

[The interferon-inducing activity of the Lassa virus in the body of inbred mice].

The level and the time course of accumulation of endogenous interferon were studied in the blood sera of mice experimentally infected with Lassa virus by the intracerebral route. The level of interferon correlated with case-fatality rate of the animals which may be important for the understanding of the pathogenesis of arenavirus infections.

[Characteristics of the immune response in mice immunized with the inactivated Lassa virus].

The influence of humoral and cell-mediated immunity on the formation of resistance in mice immunized with inactivated Lassa virus was evaluated. As a result of immunization, both nonspecific and specific immune response developed, the main rule in the formation of resistance belonging to specific immunity.

[Chronic infection of BHK-21 cells by the Machupo virus. The development of the system and properties of the virus produced by cells of this line].

A new model of chronic infection of BHK-21 cells with Machupo virus has been obtained. The chronically infected culture of BHK-21-M cells differed morphologically from the control culture by the presence of giant mono- and multinuclear forms with granular cytoplasm and by an increase in the number of small rounded cells. In the system of BHK-21-M cells, the persisting virus was produced permanently but the per cent of infected cells determined by indirect immunofluorescence and the infectious centre method was not high (12-17%).

[Sedimentation analysis of the virion RNA of Machupo virus].

Pichinde and Machupo viruses labeled with 3H-uridine were concentrated and purified by different methods. The buoyant density of the viruses was for Pichinde 1.14-1.

[Accumulation of certain arenaviruses in transplantable VERO and BHK-21 cells].

Production of Lassa and Machupo viruses in Vero and BHK-21 cells was studied in relation to various conditions of the infected cell cultivation and as a function of different multiplicities of infection. The highest titers (expressed in PFU/ml) were obtained when the cells were grown in roller bottles with daily changes of the medium. The maximum titer in Lassa virus-infected cells was over 10(6), in Machupo virus-infected cells over 10(7).

[Effect of rimantadine on the synthesis of virus-specific RNA in the culture of cells infected with Sindbis virus].

Effect of rimanthadine (alpha-methyl-I-adamantane methylamine) on the synthesis of virus specific RNA was studied in culture of cells, infected with Syndbis virus. Rimanthadine inhibits the synthesis of virus specific RNA beginning from the 3-rd hr of infection. A magnitude of inhibition from the 3-rd to the 7-th hrs of infection was practically the same and exceeded 50%.

[Identification of RNAase-sensitive 20S RNA in a cell culture infected with Sindbis virus].

RNAse-sensitive 20S RNA component with molecular weight of 0.7-10(6) is found when analysing virus-specific RNAs isolated from cultured chicken embryo fibroblasts infected with Sindbis virus by means of gradient centrifugation and polyacrylamide gel electrophoresis.