Publications by authors named "Peter Reis Bezerra"
Insights into the specificity for the interaction of the promiscuous SARS-CoV-2 nucleocapsid protein N-terminal domain with deoxyribonucleic acids.
Icaro Putinhon Caruso Vitor Dos Santos Almeida Mariana Juliani do Amaral Guilherme Caldas de Andrade Gabriela Rocha de Araújo Peter Reis Bezerra
Int J Biol Macromol
April 2022
The SARS-CoV-2 nucleocapsid protein (N) is a multifunctional promiscuous nucleic acid-binding protein, which plays a major role in nucleocapsid assembly and discontinuous RNA transcription, facilitating the template switch of transcriptional regulatory sequences (TRS). Here, we dissect the structural features of the N protein N-terminal domain (N-NTD) and N-NTD plus the SR-rich motif (N-NTD-SR) upon binding to single and double-stranded TRS DNA, as well as their activities for dsTRS melting and TRS-induced liquid-liquid phase separation (LLPS). Our study gives insights on the specificity for N-NTD(-SR) interaction with TRS.
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- Coronaviruses, particularly hCoV-HKU1, are under scrutiny due to their potential to cause mild to severe respiratory illness, as highlighted by the Covid-19 pandemic.
- The N-terminal domain of the nucleocapsid protein (N-NTD) plays a crucial role in regulating viral genome transcription and assembly by binding to specific RNA sequences.
- This study provides resonance assignments for the N-NTD of HKU1-CoV, offering insights into its structural and functional properties.