Comparison Study of PVD Coatings: TiN/AlTiN, TiN and TiAlSiN Used in Wood Machining.

In this paper, we analyze the possibilities of the protection of tools for wood machining with PVD (Physical Vapor Deposition) hard coatings. The nanolayered TiN/AlTiN coating, nanocomposite TiAlSiN coatings, and single layer TiN coating were analyzed in order to use them for protection of tools for wood machining. Both nanostructured coatings were deposited in an industrial magnetron sputtering system on the cutting blades made of sintered carbide WC-Co, while TiN single layer coating was deposited by evaporation using thermionic arc.

ToF-SIMS Depth Profiling of Metal, Metal Oxide, and Alloy Multilayers in Atmospheres of H, CH, CO, and O.

The influence of the flooding gas during ToF-SIMS depth profiling was studied to reduce the matrix effect and improve the quality of the depth profiles. The profiles were measured on three multilayered samples prepared by PVD. They were composed of metal, metal oxide, and alloy layers.

Influence of Different Types of Cemented Carbide Blades and Coating Thickness on Structure and Properties of TiN/AlTiN and TiAlN/a-C:N Coatings Deposited by PVD Techniques for Machining of Wood-Based Materials.

The influence of different types of cemented carbide blades and thickness of TiAlN/a-C:N and TiN/AlTiN protective coatings used in the wood industry on cutting performance has been studied. Three types of WC-Co cemented carbide blades with different cobalt content were used in the study. The thicknesses of both types of coatings were ~2 and ~5 µm.

Microfluidic Flow Injection Immunoassay System for Algal Toxins Determination: A Case of Study.

A novel flow injection microfluidic immunoassay system for continuous monitoring of saxitoxin, a lethal biotoxin, in seawater samples is presented in this article. The system consists of a preimmobilized G protein immunoaffinity column connected in line with a lab-on-chip setup. The detection of saxitoxin in seawater was carried out in two steps: an offline incubation step (competition reaction) performed between the analyte of interest (saxitoxin or Ag, as standard or seawater sample) and a tracer (an enzyme-conjugated antigen or Ag*) toward a specific polyclonal antibody.

Development of a folic acid molecularly imprinted polymer and its evaluation as a sorbent for dispersive solid-phase extraction by liquid chromatography coupled to mass spectrometry.

This work shows the development of a molecularly imprinted polymer to determine folic acid (FA) in food extracts by using dispersive solid-phase extraction and liquid chromatography coupled to mass spectrometry (LC-MS). Herewith, combinations of monomers (methacrylic acid (MAA), 4-vinylpyridine (4VPy) and vinylbenzyl trimethylammonium chloride (VBTMAC)) and crosslinkers (ethylene glycol dimethacrylate (EGDMA) and divinyl benzene (DVB)) were tested in appropriate solvents. Isotherm tests revealed that the MIP with the highest affinity was obtained by combining VBTMAC and EGDMA.

A fully online sensor-equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications.

A new disposable, multiphase, microbioreactor (MBR; with a working volume of 550 μl) equipped with online sensors is presented for biotechnological screening research purposes owing to its high-throughput potential. Its design and fabrication, online sensor integration, and operation are described. During aerobic cultivation, sufficient oxygen supply is the most important factor that influences growth and product formation.

Towards microbioprocess control: an inexpensive 3D printed microbioreactor with integrated online real-time glucose monitoring.

Bioprocessing is of crucial importance in pharmaceutical, biofuel, food and other industries. Miniaturization of bioprocesses into microbioreactors allows multiplexing of experiments as well as reduction of reagent consumption and labour-intensity. A crucial part of the research within microbioreactors is biochemical analysis of product, byproduct and substrate concentrations that currently heavily relies on large analytical equipment.

Multi-function microfluidic platform for sensor integration.

The limited availability of metabolite-specific sensors for continuous sampling and monitoring is one of the main bottlenecks contributing to failures in bioprocess development. Furthermore, only a limited number of approaches exist to connect currently available measurement systems with high throughput reactor units. This is especially relevant in the biocatalyst screening and characterization stage of process development.

Determination of stability characteristics for electrochemical biosensors via thermally accelerated ageing.

Biosensors are devices that are prone to ageing; this phenomenon can be characterized as a decrease in signal over time. Biosensor stability is of a crucial importance for commercial success and as biosensors are presently being applied to an increasing and variety of applications. Stability characteristics related to shelf life, reusability and/or continuous use stability are often poorly investigated or unreported in literature, yet are important factors.