Development of pseudo-linear gradient elution for high-throughput resin selectivity screening in RoboColumn Format.

Rapid development of chromatographic processes relies on effective high-throughput screening (HTS) methods. This article describes the development of pseudo-linear gradient elution for resin selectivity screening using RoboColumns . It gives guidelines for the implementation of this HTS method on a Tecan Freedom EVO robotic platform, addressing fundamental aspects of scale down and liquid handling.