Lipase-Catalyzed Synthesis of Renewable Plant Oil-Based Polyamides.

Enzyme catalyzed synthesis of renewable polyamides was investigated using lipase B. A fatty acid-derived AB-type functional monomer, having one amine and one methyl ester functionality, was homopolymerized at 80 and 140 °C. Additionally, the organobase 1,5,7-triazabicyclo[4.

Streamlined Preparation of Immobilized Lipase B.

lipase B (CalB) was efficiently expressed (6.2 g L) in by utilizing an N-terminal tag cassette and the XylS/ expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.

Exploring water as building bricks in enzyme engineering.

A novel enzyme engineering strategy for accelerated catalysis based on redesigning a water network through protein backbone deshielding is presented. Fundamental insight into the energetic consequences associated with the design is discussed in the light of experimental results and computer simulations. Using water as biobricks provides unique opportunities when transition state stabilisation is not easily attained by traditional enzyme engineering.