Successful pregnancy with intracytoplasmic sperm injection after bacterial contamination of embryo culture in in vitro fertilization: a case report.

Background: Bacterial infection of embryo culture medium is rare but may be detrimental. The main source of embryo culture contamination is semen. Assisted reproduction centers currently lack consensus regarding the methods for preventing and managing embryo culture infection.

The importance of cytoplasmic strings during early human embryonic development.

During human fertilisation (IVF) treatments, embryologists attempt to select the most viable embryos for embryo transfer (ET). Previously, embryos were evaluated based on light microscopic morphological parameters. However, this is currently accomplished by morphokinetic analysis of time-lapse recordings.

Intracytoplasmic sperm injection does not improve the outcome of IVF treatments in patients with advanced maternal age or low oocyte number: A randomized controlled trial.

Introduction: Intracytoplasmic sperm injection (ICSI) was introduced to achieve fertilization in cases of severe male factor infertility. However, ICSI is often used in cases of non-male factor infertility, such as advanced maternal age or low oocyte number, but the clinical benefit of the method in these indications has not been proven.

Material And Methods: A prospective randomized study was conducted in a university clinic between 2018 and 2020.

An annotated human blastocyst dataset to benchmark deep learning architectures for in vitro fertilization.

Medical Assisted Reproduction proved its efficacy to treat the vast majority forms of infertility. One of the key procedures in this treatment is the selection and transfer of the embryo with the highest developmental potential. To assess this potential, clinical embryologists routinely work with static images (morphological assessment) or short video sequences (time-lapse annotation).

[Is bacteriological screening of semen samples before in vitro fertilization treatment justified?].

The number of couples seeking assisted reproductive technologies is increasing worldwide. The question of whether routine bacteriological screening of semen is necessary during the investigation and treatment of infertility is controversial. The semen sample often contains bacteria even if the hygiene rules for collection are followed.

[The pioneering case of ovarian cryopreservation in childhood in Hungary].

With the development of the paediatric oncohaematological care and improving healing results, the focus on survival with high quality of life increases. Some oncohaematological treatments have a high gonadotoxicity and can cause infertility, therefore the fertility preservation is gaining ground worldwide. Most of the fertility preservation procedures are not yet available in childhood in Hungary.

Prospective-randomized study comparing clinical outcomes of IVF treatments where embryos were cultured individually or in a microwell group culture dish.

Culturing embryos together in a microdrop of media may improve embryo quality, based on the results of animal studies, however individual identification of the embryos in such a system is not possible. The microwell group culture dish contains 9 or 16 microwells with a minimal well-to-well distance and a specific well morphology that facilitates paracrine and autocrine effects. The microwell group culture dish enables individual identification of the embryos while providing the environment that comes with similar benefits as group culture.

Embryo density may affect embryo quality during in vitro culture in a microwell group culture dish.

Purpose: Culturing embryos in groups is a common practice in mammalian embryology. Since the introduction of different microwell dishes, it is possible to identify oocytes or embryos individually. As embryo density (embryo-to-volume ratio) may affect the development and viability of the embryos, the purpose of this study was to assess the effect of different embryo densities on embryo quality.

[First attempts in the introduction of cryopreservation of ovarian tissues].

Introduction: The oncological treatment may damage ovarian function. To prevent this, it is possible to cryopreserve the ovarian tissue, and to keep the samples for long-term storage. The frozen-thawed tissue could be retransplanted after chemo- or radiotherapy.

Giant oocytes in human in vitro fertilization treatments.

Purpose: Giant oocytes are potential sources of chromosomal abnormalities and should thus never be used in in vitro fertilization and embryo transfer (IVF-ET) procedures. The presence of giant oocytes may indicate the efficiency of the ovarian stimulation and can refer to the quality of sibling oocytes.

Methods: IVF cycles performed between January 2008 and November 2013 (n = 1521) were divided into two groups: Giant Oocyte Group (GO Group) contained cycles with at least one giant oocyte in the cohort of the retrieved oocytes (n = 37), Normal Group contained cycles with no giant oocytes (n = 1484).

Effect of hyaluronan-enriched embryo transfer medium on IVF outcome: a prospective randomized clinical trial.

Purpose: This prospective randomized study reports the effect of hyaluronan-enriched embryo transfer media on the outcome of in vitro fertilization and embryo transfer (IVF-ET) treatments.

Methods: A total of 581 IVF-ET cycles were included in this study. In the Hyaluronan (HA) group (n = 290), embryos were transferred from hyaluronan-enriched transfer medium.

Human oocytes containing large cytoplasmic vacuoles can result in pregnancy and viable offspring.

The effect of oocyte dysmorphism on further embryo development is controversial. It is generally accepted that serious oocyte abnormalities can have a negative effect on further fertilization and development. A couple reported to the clinic following 2 years of infertility and underwent five IVF/intracytoplasmic sperm injection treatments due to severe male factor infertility.

Impact of GnRH analogues on oocyte/embryo quality and embryo development in in vitro fertilization/intracytoplasmic sperm injection cycles: a case control study.

Background: Despite the clinical outcomes of ovarian stimulation with either GnRH-agonist or GnRH-antagonist analogues for in vitro fertilization (IVF) being well analysed, the effect of analogues on oocyte/embryo quality and embryo development is still not known in detail. The aim of this case-control study was to compare the efficacy of a multiple-dose GnRH antagonist protocol with that of the GnRH agonist long protocol with a view to oocyte and embryo quality, embryo development and IVF treatment outcome.

Methods: Between October 2001 and December 2008, 100 patients were stimulated with human menopausal gonadotrophin (HMG) and GnRH antagonist in their first treatment cycle for IVF or intracytoplasmic sperm injection (ICSI).

[Use of biochemical markers to predict the outcome of pregnancies conceived by in vitro fertilization].

Background: Pregnancies obtained after in vitro fertilization and embryo transfer are at increased risk for an adverse outcome compared with women who conceive naturally. Multiple gestations also occur more frequently after in vitro fertilization. Therefore, there is a need for markers that accurately detect the establishment of pregnancy and predict its outcome as early as possible, allowing for modification of monitoring and treatment if required.

Luteal-phase inhibin A and follicular-phase inhibin B levels are not characteristic of patients with an elevated LH-to-FSH ratio.

Purpose: To test whether serum inhibin levels are related to differences in gonadotropin concentrations between patients with an elevated LH-to-FSH ratio (ELF patients) and controls.

Methods: 32 ELF patients were matched with controls by age, body mass index (BMI), and cycle length.

Results: No statistically significant difference was found in follicular-phase inhibin B levels or midluteal inhibin A levels between cases and controls.

[In vitro fertilization at our department. A decade's work in figures and facts (1994-2003)].

Unlabelled: We report here on the first decade of in vitro fertilization (IVF) treatments performed at a newly established clinical infertility and assisted reproductive unit.

Objective: We present the number of treatment cycles, the distribution of treatment modalities ("classical" IVF and intracytoplasmic sperm injection, ICSI) and success rates, and relate them to national and international data.

Methods: During the last decade, ICSI was introduced gradually and is now used routinely at our department.

Early pronuclear breakdown is a good indicator of embryo quality and viability.

Objective: To examine whether the timing of pronuclear breakdown can be a predictor of embryo quality and viability.

Design: Retrospective comparison of the development and quality of early and late developing zygotes.

Setting: Infertility and endocrinology unit in a university hospital.

Prognostic value of serum CA-125 measurements on stimulation day 1 and on the day of oocyte pickup in the prediction of IVF treatment outcome.

Purpose: To assess whether serum levels of CA-125, a proposed marker of endometrial receptivity, are associated with pregnancy after IVF.

Methods: 42 IVF pregnancies were matched with nonpregnant controls. Twenty-eight additional unmatched pregnancies were included for the comparison of pregnancy outcomes.

Catheter type does not affect the outcome of intrauterine insemination treatment: a prospective randomized study.

Objective: To compare the main outcome of IUI with the Gynetics catheter (Gynetics Medical Products, Hamont-Achel, Belgium) or the Makler cannula (Sefi-Medical Instruments, Haifa, Israel).

Design: Prospective, randomized study.

Setting: Infertility and endocrinology unit in a university hospital.

[First case of successful pregnancy after preimplantation genetic diagnosis].

Unlabelled: Preimplantation genetic diagnosis is introduced for prevention of genetic disorders. The combination of in vitro fertilization technique and single cell molecular genetic diagnosis allows only unaffected embryos to be selected for embryotransfer, providing a healthy pregnancy and so also avoiding the need for its possible termination.

Case Report: The authors report the first successful case of the clinical application of preimplantation genetic diagnosis in Hungary, resulting the birth of an unaffected baby.