Treatment of root canal biofilms of Enterococcus faecalis with ozone gas and passive ultrasound activation.

Introduction: Biofilms of resistant species such as Enterococcus faecalis pose a major challenge in the treatment of root canals with established periapical disease. This study examined the effects of gaseous ozone delivered into saline on biofilms of E. faecalis in root canals of extracted teeth with and without the use of passive ultrasonic agitation.