Publications by authors named "Peter Broad"
Background: Induced sputum is used to sample inflammatory cells, predominantly neutrophils and macrophages, from the airways of COPD patients. The author's aim was to identify candidate genes associated with the degree of airflow obstruction and the extent of emphysema by expression profiling, and then to confirm these findings for selected candidates using PCR and protein analysis.
Methods: Two sputum studies were performed in Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage 2-4 COPD ex-smokers from the Evaluation of COPD Longitudinally to Identify Predictive Surrogate Endpoints (ECLIPSE) cohort.
ATP is one of the substrates of luciferase. ATP concentrations can be measured by quantitating the light output from a luciferase reaction. As kinases also use ATP, it is possible to assay kinase activity through the loss of luminescence in a coupled luciferase reaction.
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- The rate and nature of a cell's response to outside signals is influenced by intracellular regulators that modulate the activity of signaling proteins.
- The study focused on the pheromone-response pathway in the fission yeast Schizosaccharomyces pombe, leveraging its simplicity and genetic accessibility.
- Mutants defective in signaling regulation were identified, including known regulators and the novel role of MAP kinase phosphatase Pmp1 in pheromone signaling, expanding understanding of this pathway.