In situ detection and viability assessment of target microorganisms.

Rapid detection and viability assessment of pathogenic microorganisms, without the need for pre-enrichment steps, is critical in clinical microbiology, food safety, environmental quality assessment, and biosecurity. We demonstrate a powerful analytical concept and the related platform that enable in situ rapid detection, separation, sensitive quantification, and viability assessment of targeted microorganisms (bacteria and fungi) from minimally processed samples. This is based on a novel integration of magneto-affine selection and electrical impedance assay.

Direct, Rapid Detection of Pathogens from Urine Samples.

The problem of rapidly detecting pathogens directly from clinical samples poses significant analytical challenges. Addressing this issue in relation to urinary tract infections, we propose an effective protocol and related immunomagnetic test kits enabling versatile screening for the presence of pathogenic bacteria in unprocessed urine samples. To achieve this, the components of a typical immunomagnetic separation protocol were optimized towards the sensitive assessment of the aggregates formed out of immunomagnetically tagged target pathogens collected from clinical samples.

Parkinson's Disease and SARS-CoV-2 Infection: Particularities of Molecular and Cellular Mechanisms Regarding Pathogenesis and Treatment.

Accumulating data suggest that chronic neuroinflammation-mediated neurodegeneration is a significant contributing factor for progressive neuronal and glial cell death in age-related neurodegenerative pathology. Furthermore, it could be encountered as long-term consequences in some viral infections, including post-COVID-19 Parkinsonism-related chronic sequelae. The current systematic review is focused on a recent question aroused during the pandemic's successive waves: are there post-SARS-CoV-2 immune-mediated reactions responsible for promoting neurodegeneration? Does the host's dysregulated immune counter-offensive contribute to the pathogenesis of neurodegenerative diseases, emerging as Parkinson's disease, in a complex interrelation between genetic and epigenetic risk factors? A synthetic and systematic literature review was accomplished based on the "Preferred Reporting Items for Systematic Principles Reviews and Meta-Analyses" (PRISMA) methodology, including registration on the specific online platform: International prospective register of systematic reviews-PROSPERO, no.

Oral Mucosa Changes Associated with Wearing Removable Acrylic Dentures.

The main purpose of the study was to highlight the incidence of various changes in the oral mucosa associated with wearing removable acrylic dentures. Materials and method. The study was performed on a total of 45 subjects who presented at the Prosthodontics Clinic and the Oral Rehabilitation Clinic of Craiova Faculty of Dental Medicine during January 2018-May 2020.

Statistical Aspects of Partial Edentulism in a Sample of Adults in Craiova, Romania.

The purpose of the study was to analyze the prevalence of different forms of partial edentulism and the description of the various parameters associated with this disease. Materials and Methods. The study was conducted on a total of 204 subjects who presented themselves at the Clinic of Dental Prosthetics and Oral Rehabilitation Clinic of the Faculty of Dentistry Craiova between October 2015 and June 2016.

Immunohistochemical study of experimentally drug-induced gingival overgrowth.

The increasing frequency of using in the medical practice drugs that have the potential to induce gingival overgrowth (GO) and the existence of many unknown aspects in GO etiopathogenesis have prompted us to carry out this immunohistochemical experimental animal study. We conducted a cell proliferation study by Ki67 immunostaining and a cytokeratin (CK) study using anti-pan-CK AE1∕AE3 and anti-MNF116 antibodies, investigating the differences induced by different classes of drugs that are more frequently involved in the induction of GO. The results of our study indicate that CK AE1∕AE3 plays an important role not only in normal cellular proliferation, but also in hypertrophic tissues, and can be considered a marker of the proliferative process occurring in GO.

Histopathological Study of Oral Pseudoepitheliomatous Hyperplasia.

Pseudoepitheliomatous hyperplasia, also called Heck's disease, is an epithelial, inconstant and conjunctive proliferation that develops as a response to a great variety of stimuli. It is a lesion associated to different diseases, being found in the following etiopathogenic conditions: infectious pathogenic conditions, tumoral pathogenic conditions, inflammatory pathogenic conditions. We studied oral pseudoepitheliomatous hyperplasia for which we performed a histopathological study, on a group of 47 cases of oral pseudoepitheliomatous hyperplasias, where we investigated the following: oral epithelium changes, changes in the underlying lamina propria and associated etiopathogenic conditions.

The Role Played by Growth Factors TGF-β1, EGF and FGF7 in the Pathogeny of Oral Pseudoepitheliomatous Hyperplasia.

Pseudoepitheliomatous hyperplasia is an epithelial proliferation that develops in the dermis or lamina propria. It is a lesion associated to another pathology, which appears as a response to a great variety of infectious, neoplastic, inflammatory or traumatic stimuli. The etiopathogeny of this lesion is not clear yet.

Bystander effects and compartmental stress response to X-ray irradiation in L929 cells.

Bystander effects are indirect consequences of radiation and many other stress factors. They occur in cells that are not directly exposed to these factors, but receive signals from affected cells either by gap junctions or by molecules released in the medium. Characterizing these effects and deciphering the underlying mechanisms involved in radiation-induced bystander effects are relevant for cancer radiotherapy and radioprotection.

Compartmental stress responses correlate with cell survival in bystander effects induced by the DNA damage agent, bleomycin.

Physical or chemical stress applied to a cell system trigger a signal cascade that is transmitted to the neighboring cell population in a process known as bystander effect. Despite its wide occurrence in biological systems this phenomenon is mainly documented in cancer treatments. Thus understanding whether the bystander effect acts as an adaptive priming element for the neighboring cells or a sensitization factor is critical in designing treatment strategies.

Capsaicin short term administration effect on different immune parameters.

Type 1 diabetes is an autoimmune disease affecting a higher and higher number of persons; for this reason, the study of diabetes, and its complications, has shown a major interest. In order to highlight the modifications appeared in this disease, it is essential to use a suitable model. In "Cantacuzino" NIRDMI there is a double transgenic murine model which develops a fulminating form of type 1 diabetes.

[Spinal tuberculosis or bone metastases? Case report].

Tuberculosis remains one of the most important infectious disease worldwide and skeletal form has an incidence between 3% and 10%, depending on geographic area. The thoracolumbar junction is the most commonly involved in vertebral tuberculosis; some radiologic features are specifics, but other could be indistinguishable from that of lymphoma or metastases. We discuss the case of a 80-year-old man presented with a 2-month history of thoracolumbar pain, fatigability and fever.

"Supranormal" cardiac function in athletes related to better arterial and endothelial function.

Objective: Athlete's heart is associated with left ventricular (LV) hypertrophy (LVH), and "supranormal" cardiac function, suggesting that this is a physiological process. Hypertrophy alone cannot explain increase in cardiac function, therefore, other mechanisms, such as better ventriculo-arterial coupling might be involved.

Methods: We studied 60 male (21 +/- 3 years) subjects: 27 endurance athletes, and a control group of 33 age-matched sedentary subjects.

In vitro radiosensitivity of peripheral blood lymphocytes in multiple sclerosis patients.

Purpose: To assess the in vitro radiosensitivity of peripheral blood lymphocytes of secondary progressive multiple sclerosis (MS) patients in comparison to healthy individuals.

Material And Methods: Radiosensitivity of MS patients lymphocytes to in vitro irradiation of 2 Gy 60Co gamma-rays was studied in whole blood cultures and separated peripheral blood mononuclear cell (PBMC) cultures. Chromosomal radiosensitivity was investigated by means of the G0-micronucleus (MN) assay.

Chromosomal radiosensitivity in secondary-progressive multiple sclerosis patients.

Purpose: To investigate chromosomal radiosensitivity of secondary progressive (SP) multiple sclerosis (MS) patients in comparison to a group of healthy individuals.

Material And Methods: Chromosomal radiosensitivity was assessed in vitro with the G2 assay and the G0-micronucleus (MN) assay. For the G2 assay phytohaemagglutinin (PHA) stimulated blood cultures were irradiated with a dose of 0.

Effect of pre-exposure to beta rays of tritium on some biochemical parameters measured in organs of rats subsequently irradiated with fast neutrons.

The experiment examined biological responses produced by combined sequential exposure to low-level tritium contamination, followed by challenging irradiation with fast neutrons. Modifications of endogenous antioxidant potential of different organs in rats were discussed in relation to tissue radiosensitivity. Rats pre-contaminated to 7 cGy and 35 cGy have been additionally irradiated to 1 Gy with fast neutrons.

[Traumatic scapholunate instability of ligamentous origin].

A great deal of progress has been made in recent years with respect to understanding the normal and pathological anatomy of the wrist. A careful history and physical examination continued with a critical review of the standard radiographs, supplemented by additional studies as indicated, allow the astute clinician to identify specific patterns of instability and to formulate an effective treatment program for the patients. The most frequent type of ligamentous instability of the wrist was classical defined by Linscheid (1972) as "carpal injury in which a loss of normal alignment of the carpal bones develops early or late".

Treatment of scaphoid nonunions by Russe bone grafting--a retrospective analysis.

Forty-five patients with symptomatic established delayed union and nonunion of the scaphoid were operated between January 1983 and December 1997. The Russe anterior corticocancellous bone-grafting was performed in twenty-five cases. Using a grading system, we analyzed the suitability of this method, for three types of nonunion.

Effects of mistletoe lectin I and ionizing radiation on the glucose and thymidine uptake in tumour cells in vitro.

The increased uptake of hexose by mammalian cells is considered to be a general response to stress. Nowadays, mistletoe lectin separated from the extracts of the European mistletoe (Viscum album L.) is often used in adjuvant cancer therapy.

A bone marrow peroxidation study on low-dose irradiated rats.

The aim of this work was to study the modification of some biochemical parameters induced in animals irradiated by in vivo contamination with tritiated water (HTO). Four groups of animals were chronically irradiated by ingestion over 145 d. Irradiation doses were, respectively, 4, 9, 27, and 49 cGy.

NMR study of the selective inhibition of water permeability of rat erythrocyte membrane.

The inhibition of water diffusion across the rat erythrocyte membrane was studied by NMR using two basically different types of inhibitory agents: PCMB and in vivo irradiation. The contribution of lipid and protein to water permeability revealed the inhibitory effect of each pathway. Internal contamination with tritium (25-115 mGy) reduces water permeability due to protein modifications; for doses higher than 100 mGy the lipid mediated mechanism seems also to be impaired.

[The surgical treatment of tarsometatarsal dislocations].

Based on their own experience and literature, the authors review the etiopathogenic, anatomo-clinical, diagnostic and management aspects of torso-metatarsal dislocations. The surgical treatment by open reduction or reposition-arthrodesis, as a first or secondary step, using Kirschner wire and staples, are more and more used. The early and late complications, which may aggravate the long-term functional prognosis are detailed.

Electrically induced protoplast fusion for ergosterol-producing yeast strain improvement.

Electrofusion was employed for hybrid construction in ergosterol-producing yeast strains. Some fusion products proved to be hybrid with respect to ergosterol content and to remain stable over several generations.