An engineered AAV targeting integrin alpha V beta 6 presents improved myotropism across species.

Current adeno-associated virus (AAV) gene therapy using nature-derived AAVs is limited by non-optimal tissue targeting. In the treatment of muscular diseases (MD), high doses are often required but can lead to severe adverse effects. Here, we rationally design an AAV capsid that specifically targets skeletal muscle to lower treatment doses.

Human Salmonella and Shigella infections in Moroni, the capital of Great Comoro Island (1987-1988).

Over a period of two years 98 cultures of Salmonella and 17 cultures of Shigella were isolated at the El Maarouf Hospital, Moroni, capital of the Comoros. Almost half of the isolates were from children under 5 years. Salmonella belonged to a limited range of serotypes, S.

Competitive enzyme-immunoassays using native viral antigens to discriminate between HIV-1 and HIV-2 infections.

Human immunodeficiency virus (HIV) strains can be separated into two serotypes: HIV-1 and HIV-2. In the study reported herein, we developed and evaluated competitive enzyme-immunoassays (CEIA-1 for the detection of antibody to HIV-1, CEIA-2 for the detection of antibody to HIV-2) to discriminate serologically between HIV-1 and HIV-2 infections. In most of the cases, the serotyping of known reactive serum samples was done easily with the CEIAs, showing a similar specificity to the Western blot.

Adhesive properties of enteropathogenic Escherichia coli isolated from infants with acute diarrhea in Africa.

The adhesive properties of 69 enteropathogenic Escherichia coli (EPEC) strains were studied. The strains were isolated from diarrheal stools of infants in Burundi, Africa, and identified by serotyping with 12 classical EPEC O serogroup antisera. A test for adhesion to HEp-2 cells revealed that 52% of the strains showed localized adherence and 13% diffuse adherence.

[Determination of plasma progesterone. Comparison of immunochemical luminescence (LIA) and radioimmunoassay (RIA)].

The authors describe an immunoassay technique of serum progesterone using a chemiluminescent tracer (LIA): progesterone aminobutylethylisoluminol 11-a-hemisuccinate (P-ABEI). The intrinsic characteristics of this technique were studied. The progesterone concentrations of 62 serum samples were simultaneously titrated using this LIA technique and a RIA technique.

[Shigella and Salmonella isolated in Burundi from 1980 to 1985].

Data are presented on 633 strains of Shigella, and 197 strains of Salmonella, isolated in Bujumbura, capital of Burundi, between 1980 and 1985. The epidemiology of shigellosis is dominated by the resurgence of the Shiga bacillus and by its increasing resistance against all available antibacterial drugs. Salmonellosis is caused by a limited range of serotypes, most of them, with the exception of the typhoid bacillus, becoming multiresistant.

[Human brucellosis in Burundi. Serologic survey in Rusizi Plain].

A serological survey was carried out on human brucellosis in Rusizi plain in Burundi from october 1984 to september 1985: 1,087 human sera were investigated by means of the rose bengal test and the technic of Wright's sero-agglutination. It comes out of this survey that: the aggregate prevalence of positive serology is 6.6%; such a prevalence is significantly higher in professionally at risk people (7.

[Cryptococcosis in Burundi in 1985. Report of 30 cases].

Over a period of 23 months, 30 cases of cryptococcosis have been studied in Bujumbura (Burundi). Through them, epidemiological and clinical aspects have been underlined, and attempts have been made to establish links between cryptococcosis and A.I.

[Kaposi's sarcoma in Burundi and the Central African Republic in the framework of acquired immunodeficiency syndrome (AIDS)].

The authors carried out in 1985 a survey in two French speaking States in Central Africa, namely Burundi and Central African Republic (C.A.R.

Immunogenic effect of hepatitis B vaccine in children: comparison of two- and three-dose protocols.

The immunogenic effect of hepatitis B vaccine was evaluated in 183 seronegative infants from Senegal. Seventy-two seronegative infants received two 5-micrograms doses of vaccine at a two-month interval and 111 seronegative infants received three 5-micrograms doses at one-month intervals. All the children had a booster dose one year after the first injection of vaccine.