We report a case of a suspected intestinal myiasis of a child from Southeast Argentina. Diptera larvae were sampled by a physician from the nappy worn by the child and submitted for examination and identification to the Laboratorio de Artrópodos (Universidad Nacional de Mar del Plata). Based on diagnosis of the anterior and posterior spiracles and mouthparts, the larvae were identified as the false stable fly (Fallén, 1817) (Diptera: Muscidae).
View Article and Find Full Text PDFThe olive fly, Bactrocera oleae (Rossi, 1790), is the major insect pest of olives attacking both cultivated and wild olive. Bactrocera oleae carries a primary and vertically transmitted symbiont, the bacterium Candidatus Erwinia dacicola. As any primary symbiont, it plays an important role in the reproduction and lifespan of the fly.
View Article and Find Full Text PDFChanges in environmental temperature may induce variations in thermal tolerance and sensitivity in ectotherm organisms. These variations generate plastic responses that can be analyzed by examining their Thermal Performance Curves (TPCs). Additionally, some performance traits, like locomotion, could be affected by other factors such as biological interactions (e.
View Article and Find Full Text PDFMicroorganisms present in mosquitoes and their interactions are key factors affecting insect development. Among them, Wolbachia is closely associated with the host and affects several fitness parameters. In this study, the bacterial and fungal microbiota from two laboratory Culex quinquefasciatus isolines (wild type and tetracycline-cured) were characterized by metagenome amplicon sequencing of the ITS2 and 16S rRNA genes at different developmental stages and feeding conditions.
View Article and Find Full Text PDFFruit ripening involves numerous physiological, structural, and metabolic changes that result in the formation of edible fruits. This process is controlled at different molecular levels, with essential roles for phytohormones, transcription factors, and epigenetic modifications. Fleshy fruits are classified as either climacteric or non-climacteric species.
View Article and Find Full Text PDFThe use of esterase/lipase enzymes of different origins in food industry is a widely employed strategy to enhance the formation of characteristic aromatic compounds derived from fat and diversify flavour. In the present work, we studied EstA enzyme of Enterococcus faecalis and a high purity Rhizomucor miehei lipase (Palatase). EstA was obtained recombinantly in Escherichia coli BL21 (DE3), and optimum esterase activity was detected at pH 6.
View Article and Find Full Text PDFAdaptation to different soil conditions is a well-regulated process vital for plant life. AtHB23 is a homeodomain-leucine zipper I transcription factor (TF) that was previously revealed as crucial for plant survival under salinity conditions. We wondered whether this TF has partners to perform this essential function.
View Article and Find Full Text PDFBackground: The conventional feeding management of horses is still characterized by high starch and low fibre diets, which can negatively affect horse's gastrointestinal health. Thus, the aim of this study was to compare the effects of a high-starch (HS) vs. a high-fibre (HF) diet on gut health in horses.
View Article and Find Full Text PDFGravitropism is a finely regulated tropistic response based on the plant perception of directional cues. Such perception allows them to direct shoot growth upwards, above ground, and root growth downwards, into the soil, anchoring the plant to acquire water and nutrients. Gravity sensing occurs in specialized cells and depends on auxin distribution, regulated by influx/efflux carriers.
View Article and Find Full Text PDFMost humans carry mites in the hair follicles of their skin for their entire lives. Follicular mites are the only metazoans tha continuously live on humans. We propose that Demodex folliculorum (Acari) represents a transitional stage from a host-injuring obligate parasite to an obligate symbiont.
View Article and Find Full Text PDFManure-inhabiting Mesostigmata mites are important biological control agents of pest flies. However, the biodiversity of this mite community is mainly known from Europe and America, and especially from cattle manure. This study examined the diversity and abundance of Mesostigmata mites associated with various types of manure in an (intensive) agricultural region of the Middle East, i.
View Article and Find Full Text PDFHere, the potential of laser-induced breakdown spectroscopy (LIBS) in grading calcareous rocks for the lime industry was investigated. In particular, we developed a system equipped with non-intensified detectors operating in scanning mode, defined a suitable data acquisition protocol, and implemented quantitative data processing using both partial least squares regression (PLS-R) and a multilayer perceptron (MLP) neural network. Tests were carried out on 32 samples collected in various limestone quarries, which were preliminarily analyzed using traditional laboratory X-ray fluorescence (XRF); then, they were divided into two groups for calibration and validation.
View Article and Find Full Text PDFBackground: Chronic airway diseases are prevalent and costly conditions. Interdisciplinary rehabilitation programs that include Acceptance and Commitment-based (ACT) components could be important to tackle the vicious circle linking progression of the disease, inactivity, and psychopathological symptoms.
Methods: A retrospective evaluation of routinely collected data of an interdisciplinary rehabilitation program was performed.
Over the past few decades, there has been a growing demand for genome analysis of ancient human remains. Destructive sampling is increasingly difficult to obtain for ethical reasons, and standard methods of breaking the skull to access the petrous bone or sampling remaining teeth are often forbidden for curatorial reasons. However, most ancient humans carried head lice and their eggs abound in historical hair specimens.
View Article and Find Full Text PDFEarly to Middle Miocene sea-level oscillations of approximately 40-60 m estimated from far-field records are interpreted to reflect the loss of virtually all East Antarctic ice during peak warmth. This contrasts with ice-sheet model experiments suggesting most terrestrial ice in East Antarctica was retained even during the warmest intervals of the Middle Miocene. Data and model outputs can be reconciled if a large West Antarctic Ice Sheet (WAIS) existed and expanded across most of the outer continental shelf during the Early Miocene, accounting for maximum ice-sheet volumes.
View Article and Find Full Text PDFBurial of a cadaver results in a slower decomposition rate, due to more stable below-ground temperatures and restricted access to necrophagous insects. In such circumstances, analysis of the soil mesofauna, with emphasis on mites (Acari) may be more valuable in time-of-death estimations. The production of volatile organic compounds of cadaveric decay results in changes, especially in the soil pH, which in turn would affect the abundance and diversity of the associated mites.
View Article and Find Full Text PDFThe burial of a cadaver results in reduced arthropod activity and disruptions in colonisation patterns. Here, the distribution and diversity of mite taxa was studied across decomposition stages of shallowly buried pig carcasses (Sus scrofa domesticus). In total 300 mites (88 species) were collected from three pig shallow graves compared to 129 mites (46 species) from control (bare) soil samples at the same depth.
View Article and Find Full Text PDFThe availability of L-arginine in tumours is a key determinant of an efficient anti-tumour T cell response. Consequently, increases of typically low L-arginine concentrations within the tumour may greatly potentiate the anti-tumour responses of immune checkpoint inhibitors, such as programmed death-ligand 1 (PD-L1)-blocking antibodies. However, currently no means are available to locally increase intratumoural L-arginine levels.
View Article and Find Full Text PDFThe identification of CD4 T cell epitopes is instrumental for the design of subunit vaccines for broad protection against coronaviruses. Here, we demonstrate in COVID-19-recovered individuals a robust CD4 T cell response to naturally processed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein and nucleoprotein (N), including effector, helper, and memory T cells. By characterizing 2943 S-reactive T cell clones from 34 individuals, we found that the receptor-binding domain (RBD) is highly immunogenic and that 33% of RBD-reactive clones and 94% of individuals recognized a conserved immunodominant S346-S365 region comprising nested human leukocyte antigen DR (HLA-DR)- and HLA-DP-restricted epitopes.
View Article and Find Full Text PDFIntroduction: The retrosigmoidal (RS) placement of the Bonebridge system (BB) has been advocated for cases of unfavourable anatomical or clinical conditions which contraindicate transmastoid-presigmoidal positioning. However, these disadvantageous conditions, combined with the considerable dimensions of the implant, may represent a challenge, especially for surgeons with no skull base experience. Moreover, the literature reports only limited experience concerning RS implantation of the BB system.
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