Split-Dose Administration Enhances Immune Responses Elicited by a mRNA/Lipid Nanoparticle Vaccine Expressing Respiratory Syncytial Virus F Protein.

mRNA vaccines have recently received significant attention due to their role in combating the SARS-CoV-2 pandemic. As a platform, mRNA vaccines have been shown to elicit strong humoral and cellular immune responses with acceptable safety profiles for prophylactic use. Despite their potential, industrial challenges have limited realization of the vaccine platform on a global scale.

Outcomes of the Supris Sling in an Urban Latina Population.

Objective: To analyze patient reported outcomes, safety, and efficacy of the Supris mid-urethral sling in a diverse population.

Methods: A retrospective chart review of 101 women who underwent a mid-urethral Suprissling procedure was conducted. Baseline characteristics and perioperative parameters were collected.

Multiple model-informed open-loop control of uncertain intracellular signaling dynamics.

Computational approaches to tune the activation of intracellular signal transduction pathways both predictably and selectively will enable researchers to explore and interrogate cell biology with unprecedented precision. Techniques to control complex nonlinear systems typically involve the application of control theory to a descriptive mathematical model. For cellular processes, however, measurement assays tend to be too time consuming for real-time feedback control and models offer rough approximations of the biological reality, thus limiting their utility when considered in isolation.

Bone marrow-derived progenitor cells augment venous remodeling in a mouse dorsal skinfold chamber model.

The delivery of bone marrow-derived cells (BMDCs) has been widely used to stimulate angiogenesis and arteriogenesis. We identified a progenitor-enriched subpopulation of BMDCs that is able to augment venular remodeling, a generally unexplored area in microvascular research. Two populations of BMDCs, whole bone marrow (WBM) and Lin(-)/Sca-1(+) progenitor cells, were encapsulated in sodium alginate and delivered to a mouse dorsal skinfold chamber model.

Long-term results of treatment for prostate carcinoma by staging pelvic lymph node dissection and definitive irradiation using low-dose rate temporary iridium-192 interstitial implant and external beam radiotherapy.

Background: The objective of this study was to evaluate long-term treatment outcome of definitive irradiation by using temporary interstitial implant and limited dose of external beam radiotherapy in treatment of localized prostate carcinoma.

Methods: In total, 536 patients with biopsy-proven adenocarcinoma of the prostate, classification T1-T3, underwent staging pelvic lymph node dissection and brachytherapy delivering an average tumor dose of 30 grays (Gy), supplemented by external beam radiation therapy for an additional dose of 36 Gy delivered over 4 weeks. One hundred of 536 (18%) patients had pathologic D1 disease.

Effects of corn processing and dietary fiber source on feedlot performance, visceral organ weight, diet digestibility, and nitrogen metabolism in lambs.

In Exp. 1, early-weaned Targhee and Polypay crossbred lambs (60 ewes and 66 rams; initial BW 24 +/- 1.0 kg) were used in a 2 x 3 factorial experiment to determine the effects of corn processing (whole shelled corn [WSC] or ground and pelleted corn [GC]) in combination with supplemental fiber (none [control]; soybean hulls, SBH [highly digestible]; or peanut hulls, PH [highly indigestible]) on DMI, ADG, feed efficiency, and visceral organ weight.

Temporary iridium-192 implant in the management of carcinoma of the prostate.

The controversy about the treatment of carcinoma of the prostate has increased in the last decade, with most urologists favoring radical prostatectomy rather than primary irradiation. Several reports of persistent tumors in 50% to 90% of patients after external irradiation and permanent iodine-125 implantation of the prostate have been disturbing. From July 1977 to December 1985, 200 patients with adenocarcinoma of the prostate were treated by combining bilateral pelvic lymphadenectomy and temporary interstitial iridium-192 implantation of the prostate, followed by external irradiation.

Cytoplasmic streaming in the root cortex and its role in the delivery of potassium to the shoot.

The influence of cytochalasin B (CB), a potent inhibitor of cytoplasmic streaming, on (86)Rb-labelled K(+) translocation by detopped Lycopersicon esculentum Mill., Cucumis sativus L. and Zea mays L.

Experience with the Small-Carrion penile prosthesis in the treatment of organic impotence.

Since certain animals are endowed with an os penis the concept of using penile implants was a natural development. Herein we report on our experience with the Small-Carrion penile prosthesis in 23 patients. The surgical technique is described as well as several examples of postoperative results.

The Use of an Enzyme Electrode in the Analysis of Indole-3-acetic Acid Oxidase Activity in Avena.

A flexible analytical system which allows for the continuous potentiometric monitoring of the disappearance of an electrochemical species, ferrocyanide, by the peroxidase enzyme is described. The ability of peroxidase to mediate the oxidation of indole-3-acetic acid is followed by observing the competition of indole-3-acetic acid with ferrocyanide for the peroxidase enzyme. This is accomplished by examining potentiometrically the decrease in the rate of ferrocyanide oxidation with increasing indole-3-acetic acid concentration.

Metabolism of tryptophan in petioles of coleus.

Auxin precursors retard abscission when applied to debladed petioles of Coleus blumei Benth. The d and l forms of tryptophan are equally effective in retarding abscission. Tryptamine is more effective than is tryptophan.

The production of tryptamine from tryptophan by Bacillus cereus (KVT).

1. A strain of Bacillus cereus has been isolated that can produce tryptamine when grown in a broth containing tryptophan. 2.

On the ability of Taphrina deformans to produce indoleacetic acid from tryptophan by way of tryptamine.

The metabolism of tryptophan by Taphrina deformans has been studied to confirm the reported ability of this organism to produce tryptamine. Such amine production was not observed, despite use of amine oxidase inhibitors at levels which should have resulted in the accumulation of tryptamine in the medium. It has been shown that the metabolites of tryptophan include indolepyruvic acid, indolelactic acid, tryptophol, and indoleacetic acid, and that the original report of tryptamine production must be reevaluated in light of the extraction procedures employed.