Plasma lipid levels in insulin-dependent diabetes mellitus.

Because several recent reports have indicated a high incidence of hyperlipidemia in insulin-dependent juvenile diabetes, the plasma lipid levels were measured in a population of insulin-dependent diabetic patients to determine if hyperlipidemia is necessarily associated with diabetes. Only one patient had an elevated cholesterol concentration (greater than 220 mg. per deciliter) and two patients had an elevated triglyceride concentration (greater than 140 mg.

A system for the three-dimensional construction, manipulation and display of microbiological models.

A system is described for building up serial sections into a three dimensional structure, incorporating density, that can be displayed and then further manipulated by rotation about three orthogonal axes. The initial application was to produce a computer model of a protein structure and to compare the diverse images obtained from rotation with the two dimensional images observed in related electron micrographs. To obtain sufficient contrast in the electron microscope images of protein structures, the specimens need to be stained and since this can cause some deformation of the observed images, it is also necessary to simulate ths possible effects of stain on the protein model.

A scanning electron microscope study of the caecal tonsil: the identification of a bacterial attachment to the villi of the caecal tonsil and the possible presence of lymphatics in the caecal tonsil.

A scanning electron microscope (SEM) was used to compare the proximal region (PR) and distal region (DR) of the caecum. The caecal tonsil (CT) occupied the initial 4-10 mm of the PR. Villi were present in the PR and absent from the DR.

Contact sites between lymphoid cells of the bursa of Fabricius, in vivo and in vitro.

Contact sites between lymphocytes and between lymphocytes and macrophages were demonstrated by electron microscopy in the lymphoid follicles of the bursa of Fabricius. When compared with nonspecialized regions of the cell membranes, these contact sites were characterized by a decreased intercellular distance, subplasmalemmal densities and coated pits. Microfilaments, microtubules and coated vesicles of the subjacent cytoplasm were frequently associated with these contact sites.

Stuttering: discoordination of phonation with articulation and respiration.

Complexity of phonatory and respiratory adjustments was systematically simplified in 30 adult stutterers under three speaking conditions: voiced, whispered, and articulated without phonation. Stuttering was reduced considerably when whispering and was practically eliminated when articulating silently. The possibility that stuttering consistently results from complexity of phonatory coordinations with articulation and respiration was strongly supported.

Effect of 3,4-toluenediamine on output from in situ rat Brunner's glands pouches.

Duodenal and gastric glandular mucosal damage have been observed 24 hr following single subcutaneous doses of 3,4-TDA in fed, unrestrained rats. 3,4-TDA significantly reduced secretion from in situ Brunner's glands pouches in pentobarbital anesthetized rats. The reduction in volume output with a definitely duodenal ulcerogenic dose of this compound was more than twice that observed with a minimally ulcerogenic dose, suggesting a correlation between the duodenal ulcerogenic and duodenal anti-secretory activities of this compound.

3H-uridine incorporation by small lymphocytes of tolerant rats: relationship to T and B lymphocytes.

Tissue tolerance was induced in neonatal rats by the intravenous injection of bone marrow cells from adult allogeneic rat donors. After 6 to 8 weeks, lymphoid cells from rats in which tolerance had been induced were tested for mixed lymphocyte reactivity (MLR), 3H-uridine uptake, and the relationship of uridine incorporation to B and T lymphocytes. Lymph node (LN) and spleen (SPL) cells from the adult inoculated rats showed no reactivity in the MLR or normal lymphocyte transfer reaction (NLTRx), indicating that the animals were tolerant.

Immunoglobulin-positive cells: their appearance in the mixed lymphocyte reaction.

In mixed lymphocyte cultures prepared with thoracic duct lymphocytes from allogeneic rats, approximately 20 percent of all blast cells that appeared at the end of 72 hours of incubation had surface receptors for rabbit antibody to rat immunoglobulin.

Comparison of lymphocyte populations bearing surface immunoglobulins in avian bone marrow, bursa, spleen and thymus.

Rabbit anti-chicken gamma-globulin was labeled with 125I and then incubated with cells from the bursa, thymus, spleen, and bone marrow of 4- and 8-week old birds. The same procedure was carried out on 11-week-old agammaglobulinemic chickens. Autoradiography revealed that the majority of large, medium, and small bursal lymphocytes bind the antibodies while labeled lymphocytes of each type in the spleen and thymus never exceeded 11 or 4 percent, respectively.