[Determination of chondroitin sulfate in food supplements by capillary zone electrophoresis].

Chondroitin sulfate is widely used as an ingredient in food supplements. A method of capillary zone electrophoresis for qualitative and quantitative analysis of chondroitin sulfate in food supplements has been developed. The system of capillary electrophoresis Agilent 3D CE (USA) with diode array detector (spectral range 190-400 nm, 192 nm was used to quantity), quartz capillary Agilent with effective length 56 cm (USA) (internal diameter 50 microm, temperature 25 degrees C, 30 kV, negative polarity) and 50 mM phosphate buffer (pH 3.

[Development of methods for determining acrylamide in food products by gas-liquid chromatography].

The method of determination of acrylamide in various food (milk powder, potato chips, instant coffee) by gas-liquid chromatography after pre-bromination was developed. Studies have shown the possibility of using bromination of acrylamide to give it the necessary properties for better extraction, purification and detection. Also revealed the possibility of qualitative and quantitative determine a acrylamide in food by gas-liquid chromatography with detection by electron capture detector.

[Composition and content of biologically active substances in rose hips].

The paper studies the chemical composition of the powders obtained from the pulp with the skins and seeds of fruits of wild rose hips. Research results have shown that the main fraction of the powder is dietary fiber, powder of seeds of insoluble fiber in 1,6 and 2,3 higher than in the powder of the fruit with a thin skin and pulp, respectively. The greatest amount of carbohydrates and protein found in powders and pulp of the fruit with a thin skin, and lipids predominate in the powder from the seeds.

[Methods quantitative for determination of water-soluble vitamins in premixes and fortified food products by micellar electrokinetic chromatography on short end of the capillary].

It was purposed new technique by micellar electrokinetic chromatography on short end of the capillary (capillary electrophoresis system Agilent 3D CE, DAD, quartz capillary HPCE stndrd cap 56 cm, 50 microm, 50 mM borate buffer pH=9,3, 100 mM sodium dodecil sulfate) for simultaneous determination of water-soluble vitamins (B1, B2, B6, B12, PP, B5, B9, C, B8) in fortified food products and premixes. It was observed on 6 samples of vitamin premixes and 28 samples of fortified food products using this technique. Our findings are consistent with the results of research on certain vitamins, conducted by other methods.

[Variability of the plasma proteome in healthy people (report 1)].

The last decade has seen intense development of proteomic technologies have opened new perspectives for rapid large-scale screening of biological samples in order to find biomarkers of various diseases or conditions. However, in order to adequately evaluate the possibility of using protein as a biomarker, it is necessary to know how much its concentration varies widely in healthy people. This project aims to explore the limits of the concentration of protein components of plasma in healthy people.

[The detection short chain fatty acids in foods by gas-liquid chromatography method].

On the basis of gas-liquid chromatography (GLC) technique of quantitative determination in food, and biologically active additives (BAA) to food short-chain fatty acids: acetic, propionic, butyric, isobutyric, valeric and isovaleric. For this purpose, we used the method of GLC with a plasma-ionization detection, which allowed us to determine the amount of these acids in dietary supplements, prepared on the basis of bacterial.

[Determination by high performance chromatography, steroid saponins in a biologically active food supplements containing the extract of Tribulus terrestris].

Steroidal saponins are bioactive substances of Tribulus terrestris and can be used to assess the quality of raw materials and processed products from them. For this purpose has been developed the method of qualitative and quantitative determination of steroidal saponins by high performance liquid chromatography with spectrophotometric and mass-selective detection and optimal conditions of sample preparation (70% methanol extraction with sonication and heating); also has been studied steroidal saponins composition of Tribulus terrestris (protodioscin, tribulosaponin B, metilprotodiostsin, terrestrozin H, prototribestin, gracillin and others were found).

[Determination of melamine in milk and milk-containing products by capillary zone electrophoresis].

For determination of melamine methods of reversed phase and ion-pair high-performance liquid chromatography, and also immune-enzyme analysis are used. The purpose of our work was working out of a technique of determination melamine in milk and lactiferous products method CZE. As a result of researches determination conditions melamine in foodstuff by method CZE (sensitivity from 0.

[The new method for simultaneous determination of natural and synthetic food dyes by high performance liquid chromatography].

In the food industry in Russia is currently allowed to use more than 30 different dyes. Existing approaches to monitoring their use in foods are based on spectrophotometry, thin layer chromatography and high performance liquid chromatography (HPLC). Methods of research focused on the analysis of a specific class of food dye--natural or synthetic, and can not be used in the analysis of their mixtures.

[Selection of the optimal method of extraction and determination of dye Sudan I-IV and Para Red by high-performance liquid chromatography].

Since 2004 was important the problem of determination of non-food dyes as Sudan I-IV and Para Red. Therefore it was necessary to develop reliable methodologies or adapt existing analytical methods for monitoring the presence of such dyes in food. For this purpose, we have previously proposed a method for high-performance liquid chromatography with diode-array and mass detection.

[Fusariotoxins in the food production in the Russian Federation: situation 2006-2008 years].

The results of monitoring of the fusarium mycotoxins (deoxynivalenole (DON), zearalenone (ZL), T-2 and HT-2 toxins, fumonisins B1 and B2) contents in the food wheat, barley, rye, oats and corn (2006-2008 crops) in different regions of Russia were presented. DON occurrence in the food wheat, rye and barley were 9, 4 and 0,8% accordingly. DON wasn't detected in the food oats and maize in these years.

[Application of immunochemical and chromatographic techniques for monitoring of trichothecene mycotoxins type A in grains].

A sensitive HPLC-MS technique after immunoaffinity clean-up has been developed for the simultaneous determination of T-2 and HT-2 toxins of in the food grains. The limit of detection of method was 0,002 mg/kg for T-2 toxin and 0,005 mg/kg for HT-2 toxin. Comparison of existing immunochemical and chromatographic techniques of trichothecenes type A determination in grains was made.

[Determination of dyes sudans in of raw foods].

The number of cases using of forbidden dyes (such as Sudan-I and Para Red) in European foodstuff (including foodstuff from Russia) were increased last time. In this case it was required to adapt existing analytical methods for the control of dyes Sudan I-IV and Para Red. It was used for this purpose high performance liquid chromatography with detectings on diode-array and MS detector.

[The method of the simultaneous determination of the citrinin and ochratoxin A content in cereals and cereal products].

The simplifyed method of simultaneous determination of the citrinin (CT) and ochratoxin A (OTA) in cereals is described. The extraction of mycotoxins was carried out by small volumes of solvents (water/aceton/hexane) without additional sample clean up and concentration. The extracts were analysed by HPLC using mixture of methanol/ethylacetate/phosphoric acid, pH 2.