A High-Quality Phased Genome Assembly of Stinging Nettle ( ssp. ).

Stinging nettles () have a long history of association with human civilization, having been used as a source of textile fibers, food and medicine. Here, we present a chromosome-level, phased genome assembly for a diploid female clone of from Romania. Using a combination of PacBio HiFi, Oxford Nanopore, and Illumina sequencing, as well as Hi-C long-range interaction data (using a novel Hi-C protocol presented here), we assembled two haplotypes of 574.

Information-Theoretic Modeling of Categorical Spatiotemporal GIS Data.

An information-theoretic data mining method is employed to analyze categorical spatiotemporal Geographic Information System land use data. Reconstructability Analysis (RA) is a maximum-entropy-based data modeling methodology that works exclusively with discrete data such as those in the National Land Cover Database (NLCD). The NLCD is organized into a spatial (raster) grid and data are available in a consistent format for every five years from 2001 to 2021.

Rapid Loss of Nutritional Symbionts in an Endemic Hawaiian Herbivore Radiation Is Associated with Plant Galling Habit.

Insect herbivores frequently cospeciate with symbionts that enable them to survive on nutritionally unbalanced diets. While ancient symbiont gain and loss events have been pivotal for insect diversification and feeding niche specialization, evidence of recent events is scarce. We examine the recent loss of nutritional symbionts (in as little as 1 MY) in sap-feeding Pariaconus, an endemic Hawaiian insect genus that has undergone adaptive radiation, evolving various galling and free-living ecologies on a single host-plant species, Metrosideros polymorpha within the last ∼5 MY.

Report of two distinct ribotypes in ITS sequences of (Poaceae) in western Canada and Alaska.

Background: L. (reed canary grass) is a widely occurring grass throughout the Northern Hemisphere. In North America, it is thought to consist of introduced agricultural forms from Europe as well as native populations.

Origins of the central Macaronesian psyllid lineages (Hemiptera; Psylloidea) with characterization of a new island radiation on endemic Convolvulus floridus (Convolvulaceae) in the Canary Islands.

A molecular survey of native and adventive psyllids in the central Macaronesian islands provides the first comprehensive phylogenetic assessment of the origins of the psyllid fauna of the Canary and Madeira archipelagos. We employ a maximum likelihood backbone constraint analysis to place the central Macaronesian taxa within the Psylloidea mitogenome phylogeny. The native psyllid fauna in these central Macaronesian islands results from an estimated 26 independent colonization events.

Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera).

Objectives: DNA Barcoding has proven to be a reliable method for rapid insect identification. The success of this method is based on the amplification of a specific region, the 'Folmer' barcode region at the 5´ start of the cytochrome c oxidase 1 gene (cox1), with universal primers. Previous studies showed failures of standard "universal" primers to amplify this region in psyllids.

The Hidden Secrets of Psylloidea: Biology, Behavior, Symbionts, and Ecology.

Psyllids constitute a diverse group of sap-feeding Sternorrhyncha that were relatively obscure until it was discovered that a handful of species transmit bacterial plant pathogens. Yet the superfamily Psylloidea is much richer than the sum of its crop-associated vectors, with over 4,000 described species exhibiting diverse life histories and host exploitation strategies. A growing body of research is uncovering fascinating insights into psyllid evolution, biology, behavior, and species interactions.

Effectiveness of virtual reality-based balance and gait in older adults with fear of movement: A systematic review and meta-analysis.

Objective: To summarize the current evidence from randomized controlled trials (RCTs) regarding the effectiveness of Virtual Reality (VR) training and functional mobility in older adults with fear of movement. TYPE: Systematic review and meta-analysis of randomized clinical trials.

Methodology: An electronic search was performed using PubMed, Embase, Medline, SPORTDiscus, Scopus, and CINAHL.

Chloroplast markers for the Malvaceae and the plastome of Henderson's checkermallow (Sidalcea hendersonii S.Wats.), a rare plant from the Pacific Northwest.

Objective: Sidalcea is a genus of flowering plants restricted to the west coast of North America, commonly known as checkermallows. Remarkably, of the ~ 30 recognized species, 16 are of conservation concern (vulnerable, imperilled or critically imperilled). To facilitate biological studies in this genus, and in the wider Malvaceae, we have sequenced the whole plastid genome of Sidalcea hendersonii.

Biotremology: Vibrational communication of Psylloidea.

Psyllids perform duetting via vibrational signals between genders that are important in pre-copulation species specific recognition. To date, vibrational behavior has been recorded in more than 100 species of psyllid, which is still only a small fraction of the ∼4000 described species. In this overview, we categorize the duet behavior into (1) reciprocal duets, (2) engaged duets, (3) three-way duets and (4) loose duets.

Uncovering Symbionts Across the Psyllid Tree of Life and the Discovery of a New Liberibacter Species, "" Liberibacter capsica.

Sap-feeding insects in the order Hemiptera associate with obligate endosymbionts that are required for survival and facultative endosymbionts that can potentially modify resistance to stress, enemies, development, and reproduction. In the superfamily Psylloidea, the jumping plant lice (psyllids), less is known about the diversity and prevalence of their endosymbionts compared to other sap-feeding pests such as aphids (Aphididae). To address this knowledge gap, using 16S rRNA sequencing we identify symbionts across divergent psyllid host lineages from around the world.

transect of Europe: records of willow-associated weevils (Coleoptera: Curculionoidea) from Greece to Arctic Norway, with insights from DNA barcoding.

Background: Curculionid beetles associated with willow ( spp.) were surveyed at 42 sites across Europe, from Greece (lat. 38.

transect of Europe: patterns in the distribution of willow-feeding psyllids (Hemiptera: Psylloidea) from Greece to arctic Norway.

Psyllids are oligophagous phytophagous insects with many specialist willow ( spp.) feeding species in two genera ( and ). We examine the patterns of distribution and co-occurrence of willow-feeding species at 42 willow sites across Europe forming a transect from Greece (lat.

Underwater endoscopic mucosal resection without submucosal injection (UEMR) for large colorectal polyps: A community-based series.

Background: Underwater endoscopic mucosal resection without submucosal injection (UEMR) is an appealing therapy for large colorectal polyps. However, this technique is not practiced widely and there are limited data evaluating UEMR in community settings.

Methods: The study comprised patients undergoing UEMR of large (≥20 mm) sessile colorectal lesions at a community-based center.

Mental toughness in surgeons: Is there room for improvement?

Background: Mental toughness is crucial to high-level performance in stressful situations. However, there is no formal evaluation or training in mental toughness in surgery. Our objective was to examine differences in mental toughness between staff and resident surgeons, and whether there is an interest in improving this attribute.

transect of Europe: additional leaf beetle (Chrysomelidae) records and insights from chrysomelid DNA barcoding.

Occurrence patterns of chrysomelid beetles (Coleoptera: Chrysomelidae), associated with willow ( spp.) at 42 sites across Europe, have previously been described. The sites form a transect from Greece (lat.

On the origin of orphan hybrids between and .

We report the investigation of an × population in British Columbia that is disjunct from its parents-the latter species is present locally but ecologically separated, while the former is entirely absent. To confirm hybridity, we used multivariate analysis of floral characters of field-sampled populations to ordinate phenotypes of putative hybrids in relation to those of the parental species. Microsatellite genotypes at 11 loci from 72 parental-type and putative hybrid individuals were analysed to assess evidence for admixture.

Revision of the Hawaiian psyllid genus , with descriptions of seven new species (Hemiptera, Psylloidea, Triozidae).

The endemic Hawaiian genus Caldwell, 1940 is highly distinctive due to the extremely long genal processes. In addition, some of the immatures are ornamented with extraordinary tubercles and tentacles. Two species are redescribed, and seven new species are described, bringing the total number of species in the genus to nine.

Dressed for success? Silver impregnated nanocrystalline dressing for initial treatment of giant omphalocele.

Objective: The purpose of this study was to describe outcomes and resource utilization in patients treated with twice-weekly silver impregnated (SI) nanocrystalline dressings for initial non-operative management of giant omphalocele (GO).

Methods: A retrospective review of patients with GO treated with SI dressings was undertaken. Clinical parameters, cost, and complications were recorded.

Number of nodes in sentinel lymph node biopsy for breast cancer: Are surgeons still biased?

Background And Objectives: The purpose of this study was to assess the number of lymph nodes removed at SLNB, and what factors might bias a surgeon's decision to remove additional nodes.

Methods: A prospectively maintained database was reviewed. All patients that had SLNB for primary treatment of breast cancer between January 2012 and March 2016 were identified.

Using herbarium-derived DNAs to assemble a large-scale DNA barcode library for the vascular plants of Canada.

Premise Of The Study: Constructing complete, accurate plant DNA barcode reference libraries can be logistically challenging for large-scale floras. Here we demonstrate the promise and challenges of using herbarium collections for building a DNA barcode reference library for the vascular plant flora of Canada.

Methods: Our study examined 20,816 specimens representing 5076 of 5190 vascular plant species in Canada (98%).