Induction of Immune-Stimulating Factors and Oncolysis Upon p14 Gene Transfer in Melanoma Cell Lines.

Together with an anti-tumor immune response, oncolysis using a recombinant viral vector promises to eliminate cancer cells by both gene transfer and host-mediated functions. In this study we explore oncolysis induced by nonreplicating adenoviral vectors used for p14 and interferon-β (hIFNβ) gene transfer in human melanoma cell lines, revealing an unexpected role for p14 in promoting cellular responses predictive of immune stimulation. Oncolysis was confirmed when UACC-62 (p53 wild-type) cells succumbed upon p14 gene transfer , whereas SK-Mel-29 (p53-mutant) benefitted from its combination with hIFNβ.

Ureaplasma diversum clearance in lung mice infection is mediated by neutrophils.

Pneumonia in cattle is one of the causes of morbidity rates and economic loss. The host response to lung infections caused by Ureaplasma diversum in bovines is virtually unknown. Here in the immune response was evaluated in a murine model for an experimental pulmonary infection by U.