Investigation of the microflora and the principles of treatment of infected open tissue defects.

Treatment of chronic defects of the body remains a difficult problem. Despite the achievements in reconstructive surgery, the failure incidence is high. Infection in the chronic defect region plays the leading role in the pathogenesis.

Several aspects of formation of auto-transplant and re-transplant vascular pedicle.

The purpose of the work was the simplification of technical side of autotransplantation and replantation by pre-preparation of vascular pedicle. 173 patients were observed and treated. Microsurgical autotransplantation of tissues was done in 29 cases, fingers autotransplantation in 44 cases (35 patients), fingers replantation in 66 cases (38 patients), replantation of thick parts of extremities in 34 cases (3 patients).

Possibilities of application of composite bone-soft tissue complex in reconstructive surgery.

Hereby the method of formation of bone-soft tissue complex with its future application in reconstructive surgery is presented. Treatment of spacious defects of human body with soft tissue lost as well as with bone injury is considered as critical problem in contemporary surgery. Even today, there is no final reference regarding the particular sort of flat to be applied in each specific case.

[Possibilities of a compound bone and soft tissue complex in reconstructive surgery].

The method of forming a bone and soft tissue complex for reconstructive surgery is presented. Sixty-eight transplantations of compound flaps of soft tissues, muscles and bones including ones on microvascular anastomosis were performed in 66 patients. Good functional and esthetic results were seen in 86.

[Methodological aspects of studying chemical preparations in a model of experimental influenzal infection].

Some methodologic approaches have been developed permitting most exhaustive characterization of antiviral and antitoxic activity of drugs in mice infected with toxigenic and nontoxigenic influenza A virus strains.

Lower leg replantation in children: railroad amputation.

Replantation in the lower leg, although technically feasible, still remains a complicated clinical issue in terms of the decision-making process. In children, a limb salvage or replant procedure would seem preferable; however, in crush amputations from railroad injury, a very severe trauma, difficulties in treatment should be thoroughly weighed against prosthetic possibilities: for example, only a fair or good functional outcome would justify an attempt to replant a severed shin. Unfortunately, the outcome in most cases is unpredictable at the time of initial evaluation, and even during the replantation procedure itself.

[Microsurgical autograft in children and adolescents].

Children make up 18% of 238 patients subjected to autotransplantation of tissues with the help of microsurgical technique. The reestablishment of blood circulation was reached in all the operations by making microvascular anastomoses. The results obtained show that the microsurgical autotransplantation of tissues can be successfully used in children and juveniles and is a perspective method.

[Method for the cross protection of mice in studying the antigenic variability of the influenza virus].

A modification of the method of cross protection of mice was developed for the study of influenza virus antigenic drift. This modification does not require a pre-adaptation of the virus to mouse lungs. The experiments of cross protection of immune animals carried out by the modified method demonstrated antigenic variability of the influenza A virus strains (H3N2) isolated in 1968-1983.

Epidemiology and specific prophylaxis of measles.

In studies in the USSR, a single dose of Leningrad-16 live measles vaccine induced sero-conversion in at least 90% of vaccinees, with a mean titer of serum antibody of 1:32 and high antibody levels throughout the 15 years after vaccination. When the vaccine was correctly stored, transported, and used for mass vaccination, measles-associated morbidity decreased and the circulation of measles virus was interrupted in some towns for several years. However, childhood vaccination over a long period changed the immunologic age of the population and resulted in increased measles-associated morbidity in adults.

Inactivated chromatographic influenza vaccine.

The characteristics of laboratory parameters of inactivated whole influenza virus vaccine, obtained by the purification of allantoic virus cultures on macroporous glass, are presented. The vaccine is characterized by small reactogenicity and safety, which allows it to be used in both adolescents and adults. Seroconversions to hemagglutinin have been found in 80--95% of individuals vaccinated once during the test vaccination by the preparation containing various strains of influenza A/H3N2/virus.

[Antigenic properties of influenza virus type A inactivated by ultraviolet irradiation].

The effect of ultraviolet irradiation (UVI) on influenza A virus was studied. UVI of influenza A virus purified by adsorption chromatography on porous glass (PG) was found to result in irreversible inactivation of virus. The rate of inactivation directly depended upon the degree of virus purification.

[Characteristics of the reactogenic and immunogenic properties of killed influence vaccine purified and concentrated by the method of adsorbed chromatography on silicate sorbents].

Reactogenic and immunogenic properties of killed influenza vaccine prepared of various strains of influenza virus by the method of sorption chromatography on sliciate sorbents were studied on small groups of volunteers; there proved to be a moderate reactogenic and a marked immunogenic activity of the experimental batches of the preparation. The dose, the concentration and the method of administration of the vaccine to obtain the immune response were determined. The indices of humoral and local immunity following a single vaccination were studied.

Immunogenicity of inactivated influenza vaccine, purified by adsorption chromatography on porous glas.

A chromatographic process has been developed for a mass purification and concentration of influenza virus. The final virus suspension, purified some 400 times, is characterized by its antigenic properties and protein content. The virus was killed by UV-light and the resulting vaccine tried on 345 people.