microRNA-8514-5p regulates adipokinetic hormone/corazonin-related peptide receptor to affect development and reproduction of Plutella xylostella.

Background: Dicer1 plays a crucial role in regulating the development and reproduction of insects. Knockout of Dicer1 causes pupal deformity, low eclosion and low fecundity in Plutella xylostella, but the mechanism behind this phenomenon is not clear. This study aims to identify differentially-expressed genes and miRNAs in the Dicer1-knockout strain (ΔPxDcr-1) and assess their impact on the reproduction and development of P.

Functional characteristics of Dicer genes in Plutella xylostella.

Background: Dicer is an endonuclease that belongs to the RNase III family and can specifically recognize and cleave double-stranded RNA (dsRNA). In most insects, there are two Dicer genes, Dicer-1 (Dcr-1) and Dicer-2 (Dcr-2), which are involved in the micro-RNA and small-interfering RNA pathways in many species, respectively. The function of Dicer in Plutella xylostella remains unknown.

Hsp70 and Hsp90 Elaborately Regulate RNAi Efficiency in .

Heat-shock proteins (HSPs) serve as molecular chaperones in the RNA interference (RNAi) pathway of eukaryotic organisms. In model organisms, Hsp70 and Hsp90 facilitate the folding and remodeling of the client protein Argonaute (Ago). However, the specific function of HSPs in the RNAi pathway of (L.

The Combination of and Its Engineered Strain Expressing dsRNA Increases the Toxicity against .

RNA interference (RNAi) has been developed and used as an emerging strategy for pest management. Here, an entomopathogen () was used to express the dsRNA for the control of . A vector containing a 325-bp fragment of the conserved region of arginine kinase gene () flanking in two ends with the promoter Pro3α was developed and transferred into 8010 and BMB171, and consequently engineered strains 8010AKi and BMB171AKi expressing dsRNA of were developed.

De novo transcriptome sequencing of Isaria cateniannulata and comparative analysis of gene expression in response to heat and cold stresses.

Isaria cateniannulata is a very important and virulent entomopathogenic fungus that infects many insect pest species. Although I. cateniannulata is commonly exposed to extreme environmental temperature conditions, little is known about its molecular response mechanism to temperature stress.