Variation of microbial necromass carbon and its potential relationship with humification during composting of chicken manure with and without biochar addition.

Microbial necromass carbon (MNC) is an important stable organic C component. However, the variation of MNC and its potential relationship with humus components in composting remains uncertain. During a 45-day chicken manure composting study with and without biochar, MNC ranged from 24.

CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity.

Protein aggregation caused by the disruption of proteostasis will lead to cellular cytotoxicity and even cell death, which is implicated in multiple neurodegenerative diseases. The elimination of aggregated proteins is mediated by selective macroautophagy receptors, which is termed aggrephagy. However, the identity and redundancy of aggrephagy receptors in recognizing substrates remain largely unexplored.

Mulch-derived microplastic aging promotes phthalate esters and alters organic carbon fraction content in grassland and farmland soils.

Agricultural plastic mulch is a major microplastics (MPs) source in terrestrial ecosystems. However, knowledge about the aging characteristics of mulch-derived MPs entering natural and agricultural soils and their effects on phthalate esters (PAEs) and organic carbon fractions is still limited. Black (contains black masterbatches) and white polyethylene (PE) and biodegradable (Bio, Poly propylene carbonate and Polybutylene adipate terephthalate synthetic material (PPC+PBAT)) mulch-derived MPs, at 0.

[Influence of Land Use and Land Cover Patterns on Water Quality at Different Spatio-temporal Scales in Hehuang Valley].

Based on the measured water quality data of Huangyuan County, Huzhu Tu Autonomous County, and Minhe Hui Tu Autonomous County in Hehuang Valley of Qinghai province in the normal and wet seasons, the effects of land use and land cover patterns on regional seasonal water quality were analyzed using remote sensing technology and mathematical statistics. The results showed that:① the concentrations of total nitrogen and total phosphorus in the surface water of Hehuang Valley were high. Water pollution areas (Class Ⅳ and Ⅴ) were mainly concentrated in the lower reaches of the river and the junction of tributaries.

CDK4/6 Inhibition Enhances Oncolytic Virus Efficacy by Potentiating Tumor-Selective Cell Killing and T-cell Activation in Refractory Glioblastoma.

Unlabelled: Glioblastoma (GBM) is among the most aggressive human cancers. Although oncolytic virus (OV) therapy has been proposed as a potential approach to treat GBM, it frequently fails because GBM cells are usually nonpermissive to OV. Here, we describe a dual-step drug screen for identifying chemical enhancers of OV in GBM.

The protective effects of natural product tunicatachalcone against neuroinflammation via targeting RIPK2 in microglia BV-2 cells stimulated by LPS.

Microglia-induced neuroinflammation plays a critical role in neurological diseases. At present, RIPK2 is considered to participate in inflammatory and autoimmune cellular pathways and diseases. RIPK2 is found to be a pivotal therapeutic target in neurologic disorders related to inflammation.

Synthesis of Iminoboryl -Carboranes by Lewis Base Promoted Aminoborirane-to-Iminoborane Isomerization.

The iminoboryl -carboranes (MeSi)-Cb-B≡N-R (Cb = BCH, , R = SiMe; , R = Bu) have been successfully synthesized by tetrahydrofuran (THF)-promoted isomerization from the corresponding -carborane-fused aminoboriranes Cb{BN(SiMe)R} (). The synthetic protocol of the previously reported borirane was optimized. The borirane Cb{BN(SiMe)Bu} () and the iminoboranes and were fully characterized by NMR, IR, and single-crystal X-ray diffraction analyses.

The regulation of NLRP3 inflammasome activation by CCDC50-mediated autophagy.

The assembly of the NLRP3 inflammasome can be initiated by a wide range of stimuli including exogenous infection as well as endogenous damage. Therefore, the tight regulation of the NLRP3 inflammasome is crucial for the host to resist microbial invasion and maintain homeostasis. Our recent work has identified a negative regulator of NLRP3-mediated inflammation, namely CCDC50 (coiled-coil domain containing protein 50).

Comprehensive evaluation of spent mushroom substrate-chicken manure co-composting by garden waste improvement: physicochemical properties, humification process, and the spectral characteristics of dissolved organic matter.

The performance of garden waste on spent mushroom substrate (SMS) and chicken manure (CM) co-composting efficiency and humification is unclear. Therefore, this study investigated the impact of garden waste addition on SMS-CM co-composting physicochemical properties, humification process, and the spectral characteristics of dissolved organic matter (DOM). The results showed that garden waste improved the physicochemical properties of SMS-CM co-compost, the thermophilic period was advanced 2 days, the seed germination index increased by 30.

CCDC50 suppresses NLRP3 inflammasome activity by mediating autophagic degradation of NLRP3.

The NLRP3-directed inflammasome complex is crucial for the host to resist microbial infection and monitor cellular damage. However, the hyperactivation of NLRP3 inflammasome is implicated in pathogenesis of inflammatory diseases, including inflammatory bowel disease (IBD). Autophagy and autophagy-related genes are closely linked to NLRP3-mediated inflammation in these inflammatory disorders.

Autophagy receptor CCDC50 tunes the STING-mediated interferon response in viral infections and autoimmune diseases.

DNA sensing and timely activation of interferon (IFN)-mediated innate immunity are crucial for the defense against DNA virus infections and the clearance of abnormal cells. However, overactivation of immune responses may lead to tissue damage and autoimmune diseases; therefore, these processes must be intricately regulated. STING is the key adaptor protein, which is activated by cyclic GMP-AMP, the second messenger derived from cGAS-mediated DNA sensing.

An unconventional role of an ASB family protein in NF-κB activation and inflammatory response during microbial infection and colitis.

Nuclear factor κB (NF-κB)-mediated signaling pathway plays a crucial role in the regulation of inflammatory process, innate and adaptive immune responses. The hyperactivation of inflammatory response causes host cell death, tissue damage, and autoinflammatory disorders, such as sepsis and inflammatory bowel disease. However, how these processes are precisely controlled is still poorly understood.

The Ubiquitin E3 Ligase Parkin Inhibits Innate Antiviral Immunity Through K48-Linked Polyubiquitination of RIG-I and MDA5.

Innate immunity is the first-line defense against antiviral or antimicrobial infection. RIG-I and MDA5, which mediate the recognition of pathogen-derived nucleic acids, are essential for production of type I interferons (IFN). Here, we identified mitochondrion depolarization inducer carbonyl cyanide 3-chlorophenylhydrazone (CCCP) inhibited the response and antiviral activity of type I IFN during viral infection.

A novel selective autophagy receptor, CCDC50, delivers K63 polyubiquitination-activated RIG-I/MDA5 for degradation during viral infection.

Autophagy is a conserved process that delivers cytosolic substances to the lysosome for degradation, but its direct role in the regulation of antiviral innate immunity remains poorly understood. Here, through high-throughput screening, we discovered that CCDC50 functions as a previously unknown autophagy receptor that negatively regulates the type I interferon (IFN) signaling pathway initiated by RIG-I-like receptors (RLRs), the sensors for RNA viruses. The expression of CCDC50 is enhanced by viral infection, and CCDC50 specifically recognizes K63-polyubiquitinated RLRs, thus delivering the activated RIG-I/MDA5 for autophagic degradation.

Combination of hemofiltration and peritoneal dialysis in the treatment of severe acute pancreatitis.

Objective: Our aim was to study the therapeutic effects and the mechanism of combination of hemofiltration (HF) and peritoneal dialysis (PD) in the treatment of severe acute pancreatitis (SAP).

Methods: Fifty-one cases of SAP were randomly divided into the HF+PD group (treated group, 36 patients) and the non-HF+PD group (control group, 15 patients). Both groups were treated by the same traditional methods.

Influence of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis.

Objective: To investigate the effect of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis (SAP).

Methods: Rats were randomly divided into sham-operated, model control, and dexamethasone-treated groups. At 3, 6, and 12 h after operation, the expression levels of P-selectin protein in one-third of the rats in each group were observed.

Effects of Salvia miltiorrhizae on ICAM-1, TLR4, NF-kappaB and Bax proteins expression in multiple organs of rats with severe acute pancreatitis or obstructive jaundice.

To observe the protective effects of Salvia miltiorrhizae injection on multiple organs of rats with SAP or OJ. Two hundred eighty-eight rats were used for SAP-associated experiments and OJ-associated experiments. The rats were randomly divided into sham-operated, model control and treated group.

[Combination of hemofiltration and peritoneal dialysis in the treatment of severe acute pancreatitis].

Objective: To study the therapeutic effects and its mechanism of combination of hemofiltration (HF) and peritoneal dialysis (PD) in the treatment of severe acute pancreatitis (SAP).

Methods: Forty patients with SAP were divided at random into the HF + PD group (therapeutic group, 25 patients) and the non-HF + PD group (contrast group, 15 patients). Both groups were treated by the conventional mode of therapy.