[The microscopical and HPLC identification of several" Muxiang" raw materials].

Objective: To differentiate and identify several species of "Muxiang".

Methods: The main microscopic features and HPLC chromatograms of diagnostic components were studied.

Results: Distinguishable characters were found in two methods.

Cloning and Sequence Analyzing the Gene of Trichosanthes Trypsin Inhibitor.

A DNA probe for Trichosanthes trypsin inhibitor was amplified from the cDNA library of Trichosanthes kirilowii by polymerase chain reaction. Using this probe to screen the cDNA library, a full-length cDNA sequence of the inhibitor was obtained. The open reading frame encodes a Pre-Pro-inhibitor with Pre-, Pro, and mature peptides of 24, 14, and 27 residues, respectively.