FOXO1 reshapes neutrophils to aggravate acute brain damage and promote late depression after traumatic brain injury.

Background: Neutrophils are traditionally viewed as first responders but have a short onset of action in response to traumatic brain injury (TBI). However, the heterogeneity, multifunctionality, and time-dependent modulation of brain damage and outcome mediated by neutrophils after TBI remain poorly understood.

Methods: Using the combined single-cell transcriptomics, metabolomics, and proteomics analysis from TBI patients and the TBI mouse model, we investigate a novel neutrophil phenotype and its associated effects on TBI outcome by neurological deficit scoring and behavioral tests.

[Preliminary Study on the Secretory Performance of Hypoxic Response Protein 1 (2626)].

Objective: To verify the secretory ability of the hypoxic response protein 1 (HRP1) encoded by (Mtb) 2626.

Methods: The target gene attached with His tag was amplified from the genome of Mtb standard virulence strain H37Rv. The recombinant plasmid contained the above amplified product was constructed and electroporated into (Ms) (MC 155) to construct a recombinant strain.

[Optimizing Recombineering System (pJV53) to Promote the Screening of the Mutants].

Objective: To establish a way for screening mutants through adding the screening markers into pJV53.

Methods: The sucrose counter selection gene and mutant hygromycin-resistant gene were inserted into pJV53; The recovery of the hygromycin-resistance indicated the successful homologous recombination in (Ms), which could serve as mutant screening marker; The sucrose counter selection could be used to screen the plasmid-free mutants.

Results: The recombinant plasmid pJV53- were successfully constructed.

[ Rv3084 Encodes Functional Esterase and Suppresses the Pro-inflammatory Cytokines ].

Objective: To explore the biological characteristics of the esterase LipR encoded by (MTB) Rv3084 and its immunomodulatory function .

Methods: The gene was amplified from MTB H37Rv strain to construct recombinant expression plasmid. After sequencing, the recombinant plasmid was transformed into for expression and purification of LipR protein.