Objective: To examine the prevalence of anaplastic lymphoma kinase (ALK) fusion gene in Chinese patients with non-small cell lung cancer (NSCLC).
Methods: In this study, 95 patients with NSCLC and corresponding clinical information and formalin-fixed paraffin-embedded (FFPE) tissue blocks were included. Hematoxylin & eosin (HE) staining, conventional ALK immunochemistry (IHC) staining and intercalated antibody-enhanced polymer (iAEP) IHC staining, and dual-color split fluorescence in situ hybridization (FISH) for ALK fusion gene were performed.
Objective: To investigate the vegetative tissues of Coleus forskohlii cultivated in Tongcheng, Hubei Province, and to provide useful information for its planting.
Methods: The root, stem, leaf and enlarged rhizome of Coleus forskohlii were subject to routine paraffin section and staining with safranin and fast green FCF solution before examination by light microscopy.
Results: The secondary tissue was well developed in root, and stem showed a higher percentage of cortex and pitch, and 4 large vascular bundles.