Publications by authors named "Peng Ge Zhang"
pH-Responsive DNA nanoassembly for detection and combined therapy of tumor.
Biosens Bioelectron
January 2022
Article Synopsis
- Researchers have created a new cancer nanotherapy that has high safety, stability, and minimal toxicity, activated by the tumor environment for selective imaging and treatment.
- The nanoassembly features specially designed DNA components and targeted molecules that allow it to identify and attack tumor cells effectively, releasing drugs in response to the acidic conditions typical of tumors.
- The study demonstrates that this innovative nanoprobe can detect pH changes and combine multiple therapeutic strategies, highlighting its potential for improved cancer diagnosis and treatment.
Aim: To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer.
Methods: We analyzed the presence of SP cells in different human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO III human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays.
Aim: To ascertain whether side population (SP) cells in HXO-Rb44 retinoblastoma cell line have cancer stem cell-like property in vitro and in vivo.
Methods: We analyzed and sorted SP from HXO-Rb44 retinoblastoma cell line by Hoechst 33342 staining on flow cytometry. SP and NSP cells were determined their ability of proliferation and self-renewal by SP reanalysis, soft agar assay and tumor sphere assay in vitro.
Side population (SP) cells can be used to identify putative cancer stem cells (CSC), but this technique is hampered by the requirement for an ultraviolet (UV) laser source. DyeCycle Violet reagent (DCV) is a DNA-binding dye that can be used in the common violet laser diode (VLD)-equipped flow cytometer. In this paper, we analyzed SP cells from several bladder cancer cell lines using either Hoechst 33342 or DCV staining.
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