Identification and validation of diagnostic biomarkers and immune cell abundance characteristics in bloodstream infection by integrative bioinformatics analysis.

() is an opportunistic pathogen that could cause life-threatening bloodstream infections. The objective of this study was to identify potential diagnostic biomarkers of bloodstream infection. Gene expression dataset GSE33341 was optimized as the discovery dataset, which contained samples from human and mice.

LuxS/AI-2 system facilitates the dissemination of antibiotic-resistant plasmids in Klebsiella pneumoniae.

Plasmid conjugation is a central mechanism driving the dissemination of antibiotic resistance in Klebsiella pneumoniae. However, the conjugative operon requires specific stimuli for activation. Identifying signals and elucidating the underlying mechanisms is crucial in combating plasmid spread.

The roles of cell wall inhibition responsive protein CwrA in the pathogenicity of .

The ability to form robust biofilms and secrete a diverse array of virulence factors are key pathogenic determinants of , causing a wide range of infectious diseases. Here, we characterized as a VraR-regulated gene encoding a cell wall inhibition-responsive protein (CwrA) using electrophoretic mobility shift assays. We constructed deletion mutants in the genetic background of methicillin-resistant (MRSA) and methicillin-sensitive (MSSA) strains.

Insights into small-molecule compound CY-158-11 antibacterial activity against .

Unlabelled: The widespread prevalence and dissemination of antibiotic-resistant bacteria, coupled with the diminishing supply of new antibiotics, emphasize the pressing necessity for the exploration of innovative antibacterial agents. Previously, we detailed the impact of the small-molecule compound CY-158-11 on biofilm. By hindering adhesion and PIA-mediated biofilm formation, subinhibitory concentrations of CY-158-11 exhibit antibiofilm activity toward .

As a novel prognostic model for breast cancer, the identification and validation of telomere-related long noncoding RNA signatures.

Background: Telomeres are a critical component of chromosome integrity and are essential to the development of cancer and cellular senescence. The regulation of breast cancer by telomere-associated lncRNAs is not fully known, though. The goals of this study were to describe predictive telomere-related LncRNAs (TRL) in breast cancer and look into any possible biological roles for these RNAs.

Phenotypic and genomic analysis of the hypervirulent methicillin-resistant ST630 clone in China.

Methicillin-resistant (MRSA) sequence type 630 (ST630) is a rarely reported lineage worldwide. This study aimed to trace the dissemination of the emerging MRSA ST630 clones in China and investigate their virulence potential. We collected 22 ST630-MRSA isolates from across China and performed whole-genome sequencing analysis and virulence characterization on these isolates.

CRISPR-Cas3 and type I restriction-modification team up against bla-IncF plasmid transfer in Klebsiella pneumoniae.

Objective: We explored whether the Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas and restriction-modification (R-M) systems are compatible and act together to resist plasmid attacks.

Methods: 932 global whole-genome sequences from GenBank, and 459 K. pneumoniae isolates from six provinces of China, were collected to investigate the co-distribution of CRISPR-Cas, R-M systems, and bla plasmid.

Characteristic of KPC-12, a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in the Carbapenem-Resistant ST11-KL47 Clone Background.

Background: Carbapenem-resistant (CRKP) infections are a great threat to public health worldwide. Ceftazidime-avibactam (CZA) is an effective -lactam/-lactamase inhibitors against CRKP. However, reports of resistance to CZA, mainly caused by carbapenemase (KPC) variants, have increased in recent years.

Single-cell RNA sequencing analysis to evaluate antimony exposure effects on cell-lineage communications within the Drosophila testicular niche.

The increasing production and prevalence of antimony (Sb)-related products raise concerns regarding its potential hazards to reproductive health. Upon environmental exposure, Sb reportedly induces testicular toxicity during spermatogenesis; moreover, it is known to affect various testicular cell populations, particularly germline stem cell populations. However, the cell-cell communication resulting from Sb exposure within the testicular niche remains poorly understood.

Polymyxin B and fusidic acid, a novel potent synergistic combination against and isolates with polymyxin B resistance.

The increasing prevalence of multidrug-resistant (MDR) Gram-negative bacteria and comparatively limited options of antibiotics pose a major threat to public health worldwide. Polymyxin B is the last resort against extensively resistant Gram-negative bacterial infections. However, a large number of Gram-negative bacteria exhibited high-level resistance to Polymyxin B, bringing challenges for antimicrobial chemotherapy.

A novel small-molecule compound S-342-3 effectively inhibits the biofilm formation of .

Biofilms are an important virulence factor in and are characterized by a structured microbial community consisting of bacterial cells and a secreted extracellular polymeric matrix. Inhibition of biofilm formation is an effective measure to control infection. Here, we have synthesized a small molecule compound S-342-3, which exhibits potent inhibition of biofilm formation in both MRSA and MSSA.

Genomic Analysis of Carbapenem-Resistant Hypervirulent in a Chinese Tertiary Hospital.

Background: Carbapenem-resistant hypervirulent (CR-hvKP) has become a clinical crisis and is associated with significant morbidity and mortality. The prevalence of CR-hvKP has trended upward since 2010. This study aims to describe the clinical and genomic characteristics of CR-hvKP collected from a tertiary hospital in eastern China, from August 2020 to October 2021.

A Drosophila model of gestational antimony exposure uncovers growth and developmental disorders caused by disrupting oxidative stress homeostasis.

The toxic heavy metal antimony (Sb) is ubiquitous in our daily lives. Various models have shown that Sb induces neuronal and reproductive toxicity. However, little is known about the developmental toxicity of Sb exposure during gestation and the underlying mechanisms.

Effect of place-based policy on regional economic growth: A quasi-natural experiment from China's Old Revolutionary Development Program.

Triggering economic growth is a requirement to promote human welfare and realize sustainable development in many developing countries. However, place-based policies' impact on economic growth is debatable, and its underlying mechanism is unknown. China's Old Revolutionary Development Program (ORDP) is a large-scale and novel type of place-based policy targeted at undeveloped regions in China.

Single-cell RNA sequencing reveals cell landscape following antimony exposure during spermatogenesis in Drosophila testes.

Antimony (Sb), is thought to induce testicular toxicity, although this remains controversial. This study investigated the effects of Sb exposure during spermatogenesis in the Drosophila testis and the underlying transcriptional regulatory mechanism at single-cell resolution. Firstly, we found that flies exposed to Sb for 10 days led to dose-dependent reproductive toxicity during spermatogenesis.

Characterization of Hypervirulent and Carbapenem-Resistant Isolated from Neurological Patients.

Background: Patients with neurological disorders were easier to develop severe intracranial infections caused by hypervirulent and carbapenem-resistant , leading to a distressing clinical outcome. In this study, eight hv-CRKP were isolated from neurological patients, to clarify the resistant and virulent features.

Methods: We tested the susceptibility of common antibiotics in these isolates to feature the antibiotic-resistant phenotypes.

Interactive Deep Colorization and its Application for Image Compression.

Recent methods based on deep learning have shown promise in converting grayscale images to colored ones. However, most of them only allow limited user inputs (no inputs, only global inputs, or only local inputs), to control the output colorful images. The possible difficulty lies in how to differentiate the influences of different inputs.