Generation of APN-chimeric gene-edited pigs by CRISPR/Cas9-mediated knock-in strategy.

The prevalence of porcine enteric coronaviruses (PECs), including transmissible gastroenteritis virus (TGEV), swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine delta coronavirus (PDCoV), and porcine epidemic diarrhea virus (PEDV), poses a serious threat to animal and public health. Here, we aimed to further optimize the porcine aminopeptidase N (pAPN) gene editing strategy to explore the balance between individual antiviral properties and the biological functions of pAPN in pigs. Finally, APN-chimeric gene-edited pigs were produced through a CRISPR/Cas9-mediated knock-in strategy.

Exploring the Dependence and Influencing Factors of Carbon Emissions from the Perspective of Population Development.

Working towards sustainable population development is an important part of carbon mitigation efforts, and decoupling carbon emissions from population development has great significance for carbon mitigation. Based on the construction of a comprehensive population development index (PDI), this study adopts a decoupling model to explore the dependence between carbon emissions and PDI across 30 Chinese provinces from 2001 to 2017. Then, the stochastic impacts by regression on population, affluence and technology (STIRPAT) model is used to investigate the impact of population factors on carbon emissions.

Impact of uncertainty on regional carbon peak paths: an analysis based on carbon emissions accounting, modeling, and driving factors.

Regional carbon emission paths have an important impact on the realization of China's carbon emission peak target. Due to the uncertainty of future development model, the change of carbon emissions will also face uncertainty, which will make achieving the peak target challenging. Taking Shandong, Henan, and Guangdong, three of China's most populous provinces, as examples, this study analyzed the impacts of uncertainties in carbon accounting principles, driving factors, and simulation mechanism on achieving the peak target.

Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs.

Intramuscular fat (IMF) is an important economic trait for pork quality and a complex quantitative trait regulated by multiple genes. The objective of this work was to investigate the novel transcriptional effects of a multigene pathway on IMF deposition in the longissimus dorsi (LD) muscles of pigs. Potential signaling pathways were screened by mining data from three gene expression profiles in the Gene Expression Omnibus (GEO) database.

The Possible Role of Complete Loss of Myostatin in Limiting Excessive Proliferation of Muscle Cells (C2C12) via Activation of MicroRNAs.

Myostatin (MSTN) is a member of the TGF-β superfamily that negatively regulates skeletal muscle growth and differentiation. However, the mechanism by which complete MSTN deletion limits excessive proliferation of muscle cells remains unclear. In this study, we knocked out MSTN in mouse myoblast lines using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system and sequenced the mRNA and miRNA transcriptomes.

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine Cluster.

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine (p) cluster via a CRISPR/Cas9-mediated knock-in strategy.

Precise spatial control of cavitation erosion in a vessel phantom by using an ultrasonic standing wave.

In atherosclerotic inducement in animal models, the conventionally used balloon injury is invasive, produces excessive vessel injuries at unpredictable locations and is inconvenient in arterioles. Fortunately, cavitation erosion, which plays an important role in therapeutic ultrasound in blood vessels, has the potential to induce atherosclerosis noninvasively at predictable sites. In this study, precise spatial control of cavitation erosion for superficial lesions in a vessel phantom was realised by using an ultrasonic standing wave (USW) with the participation of cavitation nuclei and medium-intensity ultrasound pulses.

[Study on the difference between components in volatile oil of Citrus reticulata before and after being processed].

Objective: To analyze the change of components in volatile oil of Citrus reticulata before and after being processed.

Methods: The volatile oil of Citrus reticulata was extracted by steam distillation and comparatively studied by GC-MS.

Results: The content of volatile of Citrus reticulata after being steamed decreased from 1.