Activation of IL-2/IL-2R pathway by Hedyotis diffusa polysaccharide improves immunotherapy in colorectal cancer.

Colorectal cancer (CRC) is a prevalent and highly malignant tumor with a limited response to immune checkpoint inhibitor-based immunotherapy. There is an urgent need for novel immunomodulatory agents to enhance the immunotherapeutic response in CRC. Hedyotis diffusa, known for its immunomodulatory properties, has long been utilized as an adjunct in cancer treatment, positioning it as a potential source for discovering new tumor immunomodulators.

Immune profiling of the macroenvironment in colorectal cancer unveils systemic dysfunction and plasticity of immune cells.

Background: Tumour immune macroenvironment is comprised of tumour and surrounding organs responding to tumourigenesis and immunotherapy. The lack of comprehensive analytical methods hinders its application for prediction of survival and treatment response in colorectal cancer (CRC) patients.

Methods: Cytometry by time-of-flight (CyTOF) and RNA-seq was applied to characterise immune cell heterogeneity in a discovery cohort including tumour, blood and intestinal architecture comprising epithelium, lamina propria, submucosa, muscularis propria of normal bowel and tumour-adjacent bowel tissues.

Dynamic heterogeneity of colorectal cancer during progression revealed clinical risk-associated cell types and regulations in single-cell resolution and spatial context.

Background: Tumor heterogeneity is contributed by tumor cells and the microenvironment. Dynamics of tumor heterogeneity during colorectal cancer (CRC) progression have not been elucidated.

Methods: Eight single-cell RNA sequencing (scRNA-seq) data sets of CRC were included.

Exploring the promotion of green technology innovation in the new energy vehicle industry: An evolutionary game analysis.

This paper aims to explore how to promote green technology innovation (GTI) among new energy vehicle (NEV) manufacturers and the strategic changes among the government, manufacturers, and consumers. From the perspective of evolutionary game theory, a tripartite evolutionary game model is established to analyze the influence of key factors on the tripartite strategies in the context of the government's willingness to subsidize gradually decreases. The main findings are as follows: (1) government subsidies provided to manufacturers better promote their willingness to participate in GTI.

High-throughput proteomics profiling-derived signature associated with chemotherapy response and survival for stage II/III colorectal cancer.

Adjuvant chemotherapy (ACT) is usually used to reduce the risk of disease relapse and improve survival for stage II/III colorectal cancer (CRC). However, only a subset of patients could benefit from ACT. Thus, there is an urgent need to identify improved biomarkers to predict survival and stratify patients to refine the selection of ACT.

Targeted Elimination of Gene in by Conjugative CRISPR-Cas9 System.

Purpose: Plasmid-borne carbapenem resistance gene accelerates the dissemination of carbapenem-resistant Enterobacteriaceae. To efficiently eliminate the -harboring plasmid and sensitize the antibiotic-resistant bacteria to meropenem, we used the CRISPR-Cas9 system for combating the carbapenem-resistant ().

Methods: A series of CRISPR-Cas9 plasmids was constructed, and specific guide RNAs(sgRNA) were designed to target the gene.

Association of Peripheral Blood Biomarkers With Response to Anti-PD-1 Immunotherapy for Patients With Deficient Mismatch Repair Metastatic Colorectal Cancer: A Multicenter Cohort Study.

Purpose: Deficient mismatch repair (dMMR) is an established biomarker for the response to the programmed cell death (PD)-1 inhibitors in metastatic colorectal cancer (mCRC). Although patients with dMMR mCRC could achieve a high incidence of disease control and favorable progression-free survival (PFS), reported response rates to PD-1 inhibitors are variable from 28% to 52%. We aimed to explore the additional predictive biomarkers associated with response to anti-PD-1 immunotherapy in patients with dMMR mCRC.

Improved Thermal Stability and Enhanced Thermoelectric Properties of p-Type BaCuTe by Doping of Cl.

Doping in semiconductors is a widely implemented strategy for manipulation of carrier concentration, which is a critical parameter to regulate the thermoelectric performance. Stoichiometric BaCuTe shows high hole concentration and unstable transport properties owing to the inherent Cu vacancy and dynamic precipitation behavior. In this work, Te has been partially substituted by Cl in BaCuTe to suppress the overhigh hole concentration.

The Predictive Value of Estrogen Receptor 1 on Adjuvant Chemotherapy in Locally Advanced Colorectal Cancer: A Retrospective Analysis With Independent Validation and Its Potential Mechanism.

To investigate the predictive biomarker value of estrogen receptor 1 (ESR1) expression in tumor tissue on adjuvant chemotherapy in curatively resected colorectal cancer (CRC). A total of 467 CRC patients in 2007-2010 were retrospectively evaluated. Clinical information and follow-up data were retrieved from hospital registries and patient files.

mA methyltransferase METTL3 suppresses colorectal cancer proliferation and migration through p38/ERK pathways.

Although many biological processes are involved in the modification of N-methyladenosine (mA), the exact role of mA in the development of malignant tumors remains unclear. Methyltransferase 3 (METTL3) is a major RNA N-methyladenosine methyltransferase. We aimed to explore the role of METTL3 in colorectal cancer (CRC) carcinogenesis and disease progression.

The complete mitochondrial genome of the hybrid grouper ♀ × ♂.

In this study, the complete mitochondrial genome of  ×  has been presented. The mitochondrial genome is 16,795 bp long and consists of 13 protein-coding genes, 2 rRNA, 22 tRNA, and a D-loop region. The phylogenetic analysis by neighbour-joining (MJ) method showed that the hybrid grouper has the closer relationship to .

The complete mitochondrial genome of the hybrid grouper ( × ) with phylogenetic consideration.

The complete mitochondrial genome of the Hybrid grouper () was presented in this study. The mitochondrial genome is 16,501 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The gene order and composition of Hybrid grouper mitochondrial genome was similar to that of most other vertebrates.